CULTURE AND PROLIFERATION OF DENDRITIC CELLS FROM MOUSE BONE MARROW PROGENITORS

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ARAB S.; MOTAMEDI M.; HAJATI J.

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Journal: JOURNAL OF ANIMAL BIOLOGY Year:2008 | Volume:1 | Issue:1 Page(s): 35-40

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Information Journal Paper

Title

CULTURE AND PROLIFERATION OF DENDRITIC CELLS FROM MOUSE BONE MARROW PROGENITORS

Author(s)

ARAB S. | MOTAMEDI M. | HAJATI J. | Issue Writer Certificate

Keywords

BONE MARROWQ3

CELL CULTUREQ2

PROLIFERATIONQ1

MATURATIONQ2

DENDRITIC CELLSQ3

Abstract

Dendritic cells (DCs) are the most potent antigen-presenting cells that induce and control the immune responses. These cells have the capacity of stimulation different types immune response (type 1 or 2) in exposure to peripheral signals. The aim of this study was DCs culture, PROLIFERATION and MATURATION from mouse BONE MARROW progenitors. Bone-marrow derived DCs (BMDCs) were cultured in the presence of GM-CSF and IL-4. After 5 days, LPS and cholera toxin were added to the culture for another 2 days. Expression of surface molecules and cytokine production in BMDC culture evaluated. The result shown that LPS stimulated DCs were matured. Exposure with CT induce IL-10 and with LPS induce IL-12 production by DCs. These fundings indicate that 7 days culture of mouce BONE MARROW progenitors can produce professional DENDRITIC CELLS and with added different components in DCs culture induce appropriate type 1 or 2 of immune responses.

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APA: Copy

ARAB, S., MOTAMEDI, M., & HAJATI, J.. (2008). CULTURE AND PROLIFERATION OF DENDRITIC CELLS FROM MOUSE BONE MARROW PROGENITORS. JOURNAL OF ANIMAL BIOLOGY, 1(1), 35-40. SID. https://sid.ir/paper/176685/en

Vancouver: Copy

ARAB S., MOTAMEDI M., HAJATI J.. CULTURE AND PROLIFERATION OF DENDRITIC CELLS FROM MOUSE BONE MARROW PROGENITORS. JOURNAL OF ANIMAL BIOLOGY[Internet]. 2008;1(1):35-40. Available from: https://sid.ir/paper/176685/en

IEEE: Copy

S. ARAB, M. MOTAMEDI, and J. HAJATI, “CULTURE AND PROLIFERATION OF DENDRITIC CELLS FROM MOUSE BONE MARROW PROGENITORS,” JOURNAL OF ANIMAL BIOLOGY, vol. 1, no. 1, pp. 35–40, 2008, [Online]. Available: https://sid.ir/paper/176685/en

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