COMPARISON OF DIFFERENT METHODS FOR DENDRITIC CELL GENERATION FROM MOUSE BONE MARROW

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AGHDAMI NASER; MOAZENI S.M.; GHARIB DOUST M.; MAHDAVI MAHDI

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Journal: Cell Journal (Yakhteh) Year:2007 | Volume:9 | Issue:2 (34) Page(s): 81-90

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Information Journal Paper

Title

COMPARISON OF DIFFERENT METHODS FOR DENDRITIC CELL GENERATION FROM MOUSE BONE MARROW

Author(s)

AGHDAMI NASER | MOAZENI S.M. | GHARIB DOUST M. | MAHDAVI MAHDI | Issue Writer Certificate

Keywords

DENDRITIC CELLQ2

MOUSE BONE MARROWQ2

DENDRITIC CELLS GENERATION METHODSQ2

Abstract

Objective: DENDRITIC CELLs (DCs) are the most effective antigen-presenting cells with many applications. However, DCs can be generated in vitro by various methods from bone marrow cells, each with varying pros and cons. Thus, we evaluated three different methods to generate DCs from mice bone marrow cells.Materials and Methods: BM cells from C57BL/6 mice were cultured in the presence of 1000U/ml of GM-CSF either with or without 500 U/ml of IL-4 for 6 days as first and second method respectively. In the third method, BM cells were cultured in bacterial plates in the presence of 200 U/ml GM-CSF for 10 days. For further maturation, the cultures were extended for two more days using 50ng/ml TNF-a. The purity of the obtained DCs, their subtypes and maturation states were determined using flow cytometric analysis and the capacity to induce the allogenic T cell proliferation was determined using [3H]-thymidine incorporation.Results: The purity of generated DCs (CD11c+) in both methods of GM-CSF plus IL-4 and culturing in bacterial plates was more than that of GM-CSF method. Culturing in bacterial plates resulted in the generation of higher number of DCs. However, DCs were resistant to maturation induction by maturation factors. Expression of maturation markers (CD86 and MHC class II) on DCs was up-regulated in the presence of IL-4 compared to the other two methods and the cells acted more efficiently in the induction of allogenic T cells proliferation. All the three methods resulted in the successful generation of myeloid (CD11b+) DCs.Conclusion: A combination of GM-CSF and IL-4 results in the generation of more pure, more mature, and functionally more effective DCs.

Cites

COMPARISON THE EFFECTS OF TWO MONOCYTE ISOLATION METHODS, PLASTIC ADHERENCE AND MAGNETIC ACTIVATED CELL SORTING METHODS, ON PHAGOCYTIC ACTIVITY OF GENERATED DENDRITIC CELLS

References

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APA: Copy

AGHDAMI, NASER, MOAZENI, S.M., GHARIB DOUST, M., & MAHDAVI, MAHDI. (2007). COMPARISON OF DIFFERENT METHODS FOR DENDRITIC CELL GENERATION FROM MOUSE BONE MARROW. CELL JOURNAL (YAKHTEH), 9(2 (34)), 81-90. SID. https://sid.ir/paper/16170/en

Vancouver: Copy

AGHDAMI NASER, MOAZENI S.M., GHARIB DOUST M., MAHDAVI MAHDI. COMPARISON OF DIFFERENT METHODS FOR DENDRITIC CELL GENERATION FROM MOUSE BONE MARROW. CELL JOURNAL (YAKHTEH)[Internet]. 2007;9(2 (34)):81-90. Available from: https://sid.ir/paper/16170/en

IEEE: Copy

NASER AGHDAMI, S.M. MOAZENI, M. GHARIB DOUST, and MAHDI MAHDAVI, “COMPARISON OF DIFFERENT METHODS FOR DENDRITIC CELL GENERATION FROM MOUSE BONE MARROW,” CELL JOURNAL (YAKHTEH), vol. 9, no. 2 (34), pp. 81–90, 2007, [Online]. Available: https://sid.ir/paper/16170/en

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