THE EVALUATION OF METHANOLIC EXTRACT OF GLYCYRRHIZA GLABRA EFFECT ON THE REPLICATION OF HERPES SIMPLEX VIRUS TYPE 1 IN VERO CELL LINE

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SABOURI GHANAD M.; MOHAMMADI A.; SAFIALLAHY S.; FARADMAL J.

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Journal: Avicenna Journal of Clinical Medicine Year:2014 | Volume:20 | Issue:4 (SN 70) Page(s): 325-332

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Title

THE EVALUATION OF METHANOLIC EXTRACT OF GLYCYRRHIZA GLABRA EFFECT ON THE REPLICATION OF HERPES SIMPLEX VIRUS TYPE 1 IN VERO CELL LINE

Author(s)

SABOURI GHANAD M. | MOHAMMADI A. | SAFIALLAHY S. | FARADMAL J. | Issue Writer Certificate

Keywords

ANTIVIRAL AGENTSQ2

GLYCYRRHIZAQ2

HERPESVIRUS 1Q2

VERO CELL LINEQ2

Abstract

Introduction & Objective: The side effects of drug consumption and also HSV resistance to drugs have been the factors attracting the researchers to herbal drugs. The major aim of the present research was in vitro assessment of possible anti-herpetic activity of GLYCYRRHIZA glabra (liquorices root) methanolic extract in more details by performing Tissue Culture Infective Dose fifty percent (TCID50) method.Materials & Methods: In this experimental study Vero cells were incubated with different concentrations of methanolic extracts of GLYCYRRHIZA glabra .Neutral red assay was performed in order to consider the appropriate concentration of the extract. Pre-treatment of Vero cells with GLYCYRRHIZA glabra extract before viral infection, incubation of HSV-1 with GLYCYRRHIZA glabra extract and treatment of vero cells with extract 1, 4, 8, 12 and 24 hours after viral infection, were exerted. TCID50 was performed in order to assess the antiviral activity of the extract. The results were analyzed after performing the experiments at least three times.Results: Pre-treatment of Vero cells with methanolic extract for two hours and incubation of virus with extract for one and two hours prior to viral infection resulted in remarkable drop in TCID50 amount by significant difference (P<0.001). Treatment of Vero cells with extract 1, 8 and 12 hours post-infection demonstrated significant changes in TCID50. We observed significant fluctuations and different efficacy of the extract between different incubation time at 1h & 4h, 1h & 24h, 4h & 8h, 4h & 12h, 8h & 24h, 12h & 24h.Conclusion: The current study resulted in more novel findings of anti herpetic activity of GLYCYRRHIZA glabra extract. Time course of extract treatment with virus- infected cells was an efficient factor. In addition, the pretreatment of virus and also Vero cells with GLYCYRRHIZA glabra extract in our experiment had important effects on the anti-viral activity of the extract.

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APA: Copy

SABOURI GHANAD, M., MOHAMMADI, A., SAFIALLAHY, S., & FARADMAL, J.. (2014). THE EVALUATION OF METHANOLIC EXTRACT OF GLYCYRRHIZA GLABRA EFFECT ON THE REPLICATION OF HERPES SIMPLEX VIRUS TYPE 1 IN VERO CELL LINE. AVICENNA JOURNAL OF CLINICAL MEDICINE (SCIENTIFIC JOURNAL OF HAMADAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES), 20(4 (SN 70)), 325-332. SID. https://sid.ir/paper/17753/en

Vancouver: Copy

SABOURI GHANAD M., MOHAMMADI A., SAFIALLAHY S., FARADMAL J.. THE EVALUATION OF METHANOLIC EXTRACT OF GLYCYRRHIZA GLABRA EFFECT ON THE REPLICATION OF HERPES SIMPLEX VIRUS TYPE 1 IN VERO CELL LINE. AVICENNA JOURNAL OF CLINICAL MEDICINE (SCIENTIFIC JOURNAL OF HAMADAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES)[Internet]. 2014;20(4 (SN 70)):325-332. Available from: https://sid.ir/paper/17753/en

IEEE: Copy

M. SABOURI GHANAD, A. MOHAMMADI, S. SAFIALLAHY, and J. FARADMAL, “THE EVALUATION OF METHANOLIC EXTRACT OF GLYCYRRHIZA GLABRA EFFECT ON THE REPLICATION OF HERPES SIMPLEX VIRUS TYPE 1 IN VERO CELL LINE,” AVICENNA JOURNAL OF CLINICAL MEDICINE (SCIENTIFIC JOURNAL OF HAMADAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES), vol. 20, no. 4 (SN 70), pp. 325–332, 2014, [Online]. Available: https://sid.ir/paper/17753/en

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