THE EFFECT OF VITAMIN E ON THE IN VITRO DIFFERENTIATION OF ADULT RAT BONE MARROW MESENCHYMAL STEM CELLS TO OSTEOBLAST DURING SODIUM ARSENITE EXPOSURE

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

SOLEIMANI MEHRANJANI M.; MAHDIYEH M.; HOSEINI S.; AZIMI A.S.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Avicenna Journal of Clinical Medicine Year:2016 | Volume:22 | Issue:4 (SN 78) Page(s): 276-285

Download Full-Text

Persian Verion

View:

908

Download:

Cites:

Information Journal Paper

Title

THE EFFECT OF VITAMIN E ON THE IN VITRO DIFFERENTIATION OF ADULT RAT BONE MARROW MESENCHYMAL STEM CELLS TO OSTEOBLAST DURING SODIUM ARSENITE EXPOSURE

Author(s)

SOLEIMANI MEHRANJANI M. | MAHDIYEH M. | HOSEINI S. | AZIMI A.S. | Issue Writer Certificate

Keywords

CELL VIABILITYQ2

MESENCHYMAL STEM CELLSQ2

OSTEOGENIC DIFFERENTIATIONQ2

SODIUM ARSENITEQ2

VITAMIN EQ2

Abstract

Introduction & Objective: SODIUM ARSENITE disturbs the differentiation of adult rat bone marrow MESENCHYMAL STEM CELLS (rMSCs) to Osteoblast through oxidative stress. We aimed to investigate the preventive effect of VITAMIN E ,a strong antioxidant ,in SODIUM ARSENITE toxicity on rMSCs differentiation to osteoblast.Materials & Methods: rMSCs were cultured in Dulbecco’s Modified Eagles Medium containing 15% Fetal Bovine Serum and divided into: control ,sodium arsenite (20 nM) ,vitamin E (50 µM) and SODIUM ARSENITE+vitamin E for 21 days in the osteogenic media containing 10% of fetal bovine serum. CELL VIABILITY ,bone matrix mineralization ,intercellular and extracellular calcium ,alkaline phosphatase activity ,DNA damage and cell morphological changes were evaluated. Data were analyzed using one- way ANOVA and Tukey's test and means were considered significantly different at P<0.05.Results: CELL VIABILITY ,bone matrix mineralization ,calcium deposition ,alkaline phosphatase activity and nuclei diameter decreased significantly in the SODIUM ARSENITE group. The mentioned parameters increased significantly in cells treated with SODIUM ARSENITE+vitamin E to the control level (P<0.05). Cytoplasmic extensions were also observed in the VITAMIN E group.Conclusions: VITAMIN E reduces SODIUM ARSENITE toxicity ,increasing OSTEOGENIC DIFFERENTIATION in rMSCs.

Cites

No record.

References

No record.

Cite

APA: Copy

SOLEIMANI MEHRANJANI, M., MAHDIYEH, M., HOSEINI, S., & AZIMI, A.S.. (2016). THE EFFECT OF VITAMIN E ON THE IN VITRO DIFFERENTIATION OF ADULT RAT BONE MARROW MESENCHYMAL STEM CELLS TO OSTEOBLAST DURING SODIUM ARSENITE EXPOSURE. AVICENNA JOURNAL OF CLINICAL MEDICINE (SCIENTIFIC JOURNAL OF HAMADAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES), 22(4 (SN 78)), 276-285. SID. https://sid.ir/paper/17807/en

Vancouver: Copy

SOLEIMANI MEHRANJANI M., MAHDIYEH M., HOSEINI S., AZIMI A.S.. THE EFFECT OF VITAMIN E ON THE IN VITRO DIFFERENTIATION OF ADULT RAT BONE MARROW MESENCHYMAL STEM CELLS TO OSTEOBLAST DURING SODIUM ARSENITE EXPOSURE. AVICENNA JOURNAL OF CLINICAL MEDICINE (SCIENTIFIC JOURNAL OF HAMADAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES)[Internet]. 2016;22(4 (SN 78)):276-285. Available from: https://sid.ir/paper/17807/en

IEEE: Copy

M. SOLEIMANI MEHRANJANI, M. MAHDIYEH, S. HOSEINI, and A.S. AZIMI, “THE EFFECT OF VITAMIN E ON THE IN VITRO DIFFERENTIATION OF ADULT RAT BONE MARROW MESENCHYMAL STEM CELLS TO OSTEOBLAST DURING SODIUM ARSENITE EXPOSURE,” AVICENNA JOURNAL OF CLINICAL MEDICINE (SCIENTIFIC JOURNAL OF HAMADAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES), vol. 22, no. 4 (SN 78), pp. 276–285, 2016, [Online]. Available: https://sid.ir/paper/17807/en

Related Journal Papers