SYNERGISTIC EFFECT OF HONEY BEE VENOM AND 1, 25-DIHYDROXYVITAMIN D3 ON THE INDUCTION OF HL-60 PRO MYELOCYTIC LEUKEMIA CELL LINE DIFFERENTIATION

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MOHSENI KOUCHESFAHANI HOMA; PARIVAR KAZEM; NABIOUNI M.; RAHIMI MARYAM

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Journal: Avicenna Journal of Clinical Medicine Year:2010 | Volume:16 | Issue:4 (SN 54) Page(s): 5-12

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Title

SYNERGISTIC EFFECT OF HONEY BEE VENOM AND 1, 25-DIHYDROXYVITAMIN D3 ON THE INDUCTION OF HL-60 PRO MYELOCYTIC LEUKEMIA CELL LINE DIFFERENTIATION

Author(s)

MOHSENI KOUCHESFAHANI HOMA | PARIVAR KAZEM | NABIOUNI M. | RAHIMI MARYAM | Issue Writer Certificate

Keywords

HONEY BEE VENOMQ3

HL-60 ACUTE PROMYELOCYTIC LEUKEMIA CELL LINE DIFFERENTIATION THERAPYQ3

1Q3

25-DIHYDROXYVITAMIN D3Q3

Abstract

Introduction & Objective: Acute promyelocytic leukemia (APL) is a kind of acute leukemia characterized by a balanced t(15, 17) translocation which fails to develop into mature cells and proliferate in an unregulated fashion. In the recent years, in addition to combinatorial chemotherapy to treat unmature cancerous cells, differentiation therapy by differentiating agents as a novel procedure is used. Since it is not possible to use high concentration of differentiating agents due to their side effects, some compounds are also used to increase their differentiating potency. In this study the effect of bee venom and 1,25-DIHYDROXYVITAMIN D3 individually and in combination on proliferation and differentiation of HL-60 cancerous cells were assayed.Materials & Methods: In this experimental in vitro study, toxic and non-toxic concentrations of HONEY BEE VENOM and 1,25- Dihydroxyvitamin D3 were determined using Trypan blue stained cell counting and MTT assay. In addition, differentiation of cells was assayed by Wright-Giemsa staining and NBT reduction test. Data were analized by one -way ANOVA test using SPSS software.Results: Our findings showed that both bee venom and 1,25-DIHYDROXYVITAMIN D3 in a dose and time dependent manner caused cell death at high concentrations and inhibited cell proliferation at lower concentration. 1,25-DIHYDROXYVITAMIN D3 at SnM concentration induced differentiation of HL-60 cells towards monocyte during 72 hours. Bee venom at 2.5 mg/ml concentration suppressed proliferation of HL-60 cells but had not any effect on their differentiation, whereas in combination with Sn M 1,25-DIHYDROXYVITAMIN D3 enhanced antiproliferative and differentiation potency of 1,25-DIHYDROXYVITAMIN D3..Conclusion: Non-toxic and inhibitory concentrations of bee venom can increase antiproliferation and differentiation potency of 1,25-DIHYDROXYVITAMIN D3.

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APA: Copy

MOHSENI KOUCHESFAHANI, HOMA, PARIVAR, KAZEM, NABIOUNI, M., & RAHIMI, MARYAM. (2010). SYNERGISTIC EFFECT OF HONEY BEE VENOM AND 1, 25-DIHYDROXYVITAMIN D3 ON THE INDUCTION OF HL-60 PRO MYELOCYTIC LEUKEMIA CELL LINE DIFFERENTIATION. AVICENNA JOURNAL OF CLINICAL MEDICINE (SCIENTIFIC JOURNAL OF HAMADAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES), 16(4 (SN 54)), 5-12. SID. https://sid.ir/paper/17827/en

Vancouver: Copy

MOHSENI KOUCHESFAHANI HOMA, PARIVAR KAZEM, NABIOUNI M., RAHIMI MARYAM. SYNERGISTIC EFFECT OF HONEY BEE VENOM AND 1, 25-DIHYDROXYVITAMIN D3 ON THE INDUCTION OF HL-60 PRO MYELOCYTIC LEUKEMIA CELL LINE DIFFERENTIATION. AVICENNA JOURNAL OF CLINICAL MEDICINE (SCIENTIFIC JOURNAL OF HAMADAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES)[Internet]. 2010;16(4 (SN 54)):5-12. Available from: https://sid.ir/paper/17827/en

IEEE: Copy

HOMA MOHSENI KOUCHESFAHANI, KAZEM PARIVAR, M. NABIOUNI, and MARYAM RAHIMI, “SYNERGISTIC EFFECT OF HONEY BEE VENOM AND 1, 25-DIHYDROXYVITAMIN D3 ON THE INDUCTION OF HL-60 PRO MYELOCYTIC LEUKEMIA CELL LINE DIFFERENTIATION,” AVICENNA JOURNAL OF CLINICAL MEDICINE (SCIENTIFIC JOURNAL OF HAMADAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES), vol. 16, no. 4 (SN 54), pp. 5–12, 2010, [Online]. Available: https://sid.ir/paper/17827/en

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