GENE CLONING OF STAPHYLOCOCCUS AUREUS PROTEIN A IN EXPRESSION VECTOR

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KAZEMI BAHRAM; KHALEGHI S.; BANDEHPOUR M.; JAHANI ABIANEH M.; MAGHEN L.; PAKZAD P.

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Journal: JOURNAL OF MICROBIAL BIOTECHNOLOGY Year:2009 | Volume:1 | Issue:2 (2) Page(s): 9-13

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Title

GENE CLONING OF STAPHYLOCOCCUS AUREUS PROTEIN A IN EXPRESSION VECTOR

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Keywords

PROTEIN A-STAPHYLOCOCCUS AUREUS-CLONINGQ3

Abstract

Background and objectives: Immunoglobulins are used for getting some information about a specific antigen and affinity chromatography is a method for immunoglobulins separation .The aim of this study was to preparation of recombinant protein A from Staphylococcus aureus for using IgG separation from human serum. Methods: The genomic DNA was extracted from Staphylococcus aureus and PCR reaction was done using a pair of primer based on protein A gene sequence. PCR product was cloned in to cloning vector. Recombinant plasmid was digested with NdeI and EcoRI restriction enzymes .The gene was purified from gel and sub cloned in to an expression vector.Results: The gene of staphylococcal protein A was amplified and PCR product contained 1435 bp sub cloned in to expression vector, successfully and confirmed.Conclusion: The recombinant plasmid is ready to express of recombinant protein for affinity chromatography column preparation.

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APA: Copy

KAZEMI, BAHRAM, KHALEGHI, S., BANDEHPOUR, M., JAHANI ABIANEH, M., MAGHEN, L., & PAKZAD, P.. (2009). GENE CLONING OF STAPHYLOCOCCUS AUREUS PROTEIN A IN EXPRESSION VECTOR. JOURNAL OF MICROBIAL BIOTECHNOLOGY, 1(2 (2)), 9-13. SID. https://sid.ir/paper/181405/en

Vancouver: Copy

KAZEMI BAHRAM, KHALEGHI S., BANDEHPOUR M., JAHANI ABIANEH M., MAGHEN L., PAKZAD P.. GENE CLONING OF STAPHYLOCOCCUS AUREUS PROTEIN A IN EXPRESSION VECTOR. JOURNAL OF MICROBIAL BIOTECHNOLOGY[Internet]. 2009;1(2 (2)):9-13. Available from: https://sid.ir/paper/181405/en

IEEE: Copy

BAHRAM KAZEMI, S. KHALEGHI, M. BANDEHPOUR, M. JAHANI ABIANEH, L. MAGHEN, and P. PAKZAD, “GENE CLONING OF STAPHYLOCOCCUS AUREUS PROTEIN A IN EXPRESSION VECTOR,” JOURNAL OF MICROBIAL BIOTECHNOLOGY, vol. 1, no. 2 (2), pp. 9–13, 2009, [Online]. Available: https://sid.ir/paper/181405/en

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