SURVEYING THE EFFICACY OF TWO METHODS OF ELISA (PRE AND POST ABSORPTION) IN THE DIAGNOSIS OF STRONGYLOIDOSIS

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KOUSHA S.; MASOUD J.

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Journal: Researcher Bulletin of Medical Sciences Year:2002 | Volume:7 | Issue:1 (27) Page(s): 81-86

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Title

SURVEYING THE EFFICACY OF TWO METHODS OF ELISA (PRE AND POST ABSORPTION) IN THE DIAGNOSIS OF STRONGYLOIDOSIS

Author(s)

KOUSHA S. | MASOUD J. | Issue Writer Certificate

Keywords

ELISAQ4

STRONGYLOIDOSISQ4

STRONGYLOIDES STERCORALISQ4

Abstract

Background: STRONGYLOIDOSIS is a prevalent disease that could lead to severe complications in case of incorrect diagnosis. ELISA is a sensitive, sufficient and serodiagnostic test for STRONGYLOIDOSIS. We have examined the efficacy of the two ELISA methods (pre and post absorption) in faculty of Hygiene, Tehran University of Medical Sciences, in 1999. Materials and methods: This clinical trial of diagnostic type has been carried out on 462 sera samples (37 of non-infected controls, 46 sera from patients with parasitologically documented STRONGYLOIDES STERCORALIS, and 378 sera from patients with other parasitic infection). In this study crude soluble antigen was obtained from filariform larvae and we performed the ELISA test in two procedures. I) ELISA test (without pre absorption) was performed on all sera. 2) After pre-incubation of sera with extracts of crossreactive parasites (after absorption of cross-reactive IgG), the ELISA test was performed on the same sera. Sensitivity, specificity, positive and negative predictive values for both of the two methods was determined. Results: This study has been carried out on 462 sera samples. Sensitivity, specificity, positive and negative predictive values for ELISA test without pre-absorption were 91, 93, 59, 99 and for ELISA test after pre-absorption were 93, 96, 73, and 99, respectively. Conclusion: The modified ELISA (after pre-absorption) is more sufficient for the diagnosis of STRONGYLOIDOSIS. Further studies are appreciated.

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APA: Copy

KOUSHA, S., & MASOUD, J.. (2002). SURVEYING THE EFFICACY OF TWO METHODS OF ELISA (PRE AND POST ABSORPTION) IN THE DIAGNOSIS OF STRONGYLOIDOSIS. RESEARCHER BULLETIN OF MEDICAL SCIENCES (PEJOUHANDEH), 7(1 (27)), 81-86. SID. https://sid.ir/paper/18262/en

Vancouver: Copy

KOUSHA S., MASOUD J.. SURVEYING THE EFFICACY OF TWO METHODS OF ELISA (PRE AND POST ABSORPTION) IN THE DIAGNOSIS OF STRONGYLOIDOSIS. RESEARCHER BULLETIN OF MEDICAL SCIENCES (PEJOUHANDEH)[Internet]. 2002;7(1 (27)):81-86. Available from: https://sid.ir/paper/18262/en

IEEE: Copy

S. KOUSHA, and J. MASOUD, “SURVEYING THE EFFICACY OF TWO METHODS OF ELISA (PRE AND POST ABSORPTION) IN THE DIAGNOSIS OF STRONGYLOIDOSIS,” RESEARCHER BULLETIN OF MEDICAL SCIENCES (PEJOUHANDEH), vol. 7, no. 1 (27), pp. 81–86, 2002, [Online]. Available: https://sid.ir/paper/18262/en

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