DETERMINATION OF GENOTYPE VARIATION OF ESCHERICHIA COLI IN WELL WATER OF TEHRAN’S PARKS BY MULTIPLEX PCR

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SOLTAN DALLAL MOHAMMAD MEHDI; SEPEHRI SAMAN; SEPEHRI SAMAN; TABATABAEI BAFROUEI AKRAM; DEILAMI KHIABANI ZAHRA

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Title

DETERMINATION OF GENOTYPE VARIATION OF ESCHERICHIA COLI IN WELL WATER OF TEHRAN’S PARKS BY MULTIPLEX PCR

Author(s)

Keywords

WATER WELLSQ2

ESCHERICHIA COLIQ2

GENETIC TESTINGQ2

DIARRHEAQ3

Abstract

Background and Aim: Water plays a major role in public health and the contaminated water has direct effect on human health. E. coli is an indicator of water pollution; different strains of E. coli such as ETEC, EHEC, EIEC and EPEC can cause DIARRHEA in children. The aim of this study is to investigate the contamination rate of well water of Tehran’s parks, and pathotyping E. coli strains which cause DIARRHEA.Materials and Methods: 156 well water samples from five different geographical parts of Tehran (north, south, east, west, and the centre) were collected aseptically. The collected samples were transferred to the laboratory in microbiology section, School of Public Health, for membrane filtrations and patotyping.Results: 90 out of 156 samples (54/5%) were contaminated with E.coli. The samples from south part were more contaminated compared to others. Out of 90 isolated E. coli only 67 were DEC, in which 42 (62.7%) were detected as EPEC, 12 (17.9%) as STEC or ETEC, 9 (13.4%) as EIEC and 4 (6%) as ETEC by PCR method.Conclusion: Presence of different biotypes of E. coli in well water of parks can be dangerous, especially for children; thus, placing warning signs and educating people in this regard should be taken seriously.

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APA: Copy

SOLTAN DALLAL, MOHAMMAD MEHDI, SEPEHRI, SAMAN, SEPEHRI, SAMAN, TABATABAEI BAFROUEI, AKRAM, & DEILAMI KHIABANI, ZAHRA. (2012). DETERMINATION OF GENOTYPE VARIATION OF ESCHERICHIA COLI IN WELL WATER OF TEHRAN’S PARKS BY MULTIPLEX PCR. RESEARCHER BULLETIN OF MEDICAL SCIENCES (PEJOUHANDEH), 16(5 (83)), 226-233. SID. https://sid.ir/paper/18335/en

Vancouver: Copy

SOLTAN DALLAL MOHAMMAD MEHDI, SEPEHRI SAMAN, SEPEHRI SAMAN, TABATABAEI BAFROUEI AKRAM, DEILAMI KHIABANI ZAHRA. DETERMINATION OF GENOTYPE VARIATION OF ESCHERICHIA COLI IN WELL WATER OF TEHRAN’S PARKS BY MULTIPLEX PCR. RESEARCHER BULLETIN OF MEDICAL SCIENCES (PEJOUHANDEH)[Internet]. 2012;16(5 (83)):226-233. Available from: https://sid.ir/paper/18335/en

IEEE: Copy

MOHAMMAD MEHDI SOLTAN DALLAL, SAMAN SEPEHRI, SAMAN SEPEHRI, AKRAM TABATABAEI BAFROUEI, and ZAHRA DEILAMI KHIABANI, “DETERMINATION OF GENOTYPE VARIATION OF ESCHERICHIA COLI IN WELL WATER OF TEHRAN’S PARKS BY MULTIPLEX PCR,” RESEARCHER BULLETIN OF MEDICAL SCIENCES (PEJOUHANDEH), vol. 16, no. 5 (83), pp. 226–233, 2012, [Online]. Available: https://sid.ir/paper/18335/en

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