DETECTION OF ENTEROINVASIVEESCHERICHIA COLI BY PCR TECHNIQUE IN CHILDREN WITH DIARRHEA

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AEIN V.; KARGAR M.; DOOSTI A.; GHOLAMI M.

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Journal Paper

Paper Information

Journal: Armaghane Danesh Year:2014 | Volume:19 | Issue:8 (91) Page(s): 727-736

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Information Journal Paper

Title

DETECTION OF ENTEROINVASIVEESCHERICHIA COLI BY PCR TECHNIQUE IN CHILDREN WITH DIARRHEA

Author(s)

AEIN V. | KARGAR M. | DOOSTI A. | GHOLAMI M. | Issue Writer Certificate

Keywords

ENTEROINVASIVEE.COLIQ2

ENTEROPATHOGENQ2

PCRQ2

ANTIBIOTIC RESISTANCEQ2

Abstract

Background & aim: EnteroinvasiveEscherichia coliis one of the important agents of invasion to intestinal epithelial cells, damage and cell death which due to dysentery. The aim of this study wastoDetection of EnteroinvasiveEscherichia coli by PCR technique from Children’s Diarrheain yasuj.Methods:This cross-sectional study was performed on 200 stool samples taken from children with diarrhea in Yasuj. After initial identification of E.coli strains by culture and biochemical tests, EIEC gene such as ipaH detected by PCR technicque and antibiotic susceptibility of isolates was evaluated by using disc diffusion (CLSI) method.Results: Out of all examined samples, 16(8%) EIEC were separated. Antibiotic susceptibility test showed that the most susceptible antibiotic is ciprofloxacin for EIEC and also most resistant antibiotic is ceftizoxime.Conclusion: Results showed that EIEC strains have a moderate prevalence than other studies in our study area. Therefore, for importance of this strain to producing dysentery, hospital-wide surveillance using molecular techniques hase been proposed in other regions of country.

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APA: Copy

AEIN, V., KARGAR, M., DOOSTI, A., & GHOLAMI, M.. (2014). DETECTION OF ENTEROINVASIVEESCHERICHIA COLI BY PCR TECHNIQUE IN CHILDREN WITH DIARRHEA. ARMAGHAN DANESH, 19(8 (91)), 727-736. SID. https://sid.ir/paper/78034/en

Vancouver: Copy

AEIN V., KARGAR M., DOOSTI A., GHOLAMI M.. DETECTION OF ENTEROINVASIVEESCHERICHIA COLI BY PCR TECHNIQUE IN CHILDREN WITH DIARRHEA. ARMAGHAN DANESH[Internet]. 2014;19(8 (91)):727-736. Available from: https://sid.ir/paper/78034/en

IEEE: Copy

V. AEIN, M. KARGAR, A. DOOSTI, and M. GHOLAMI, “DETECTION OF ENTEROINVASIVEESCHERICHIA COLI BY PCR TECHNIQUE IN CHILDREN WITH DIARRHEA,” ARMAGHAN DANESH, vol. 19, no. 8 (91), pp. 727–736, 2014, [Online]. Available: https://sid.ir/paper/78034/en

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