Optimization of Culture Media Parameters of Saccharomyces Cerevisiae PTCC 5209 for Maximized Invertase Enzyme Production

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Nejatinejad Melika; Fooladi Jamshid

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Journal Paper

Paper Information

Journal: APPLIED FOOD BIOTECHNOLOGY Year:2025 | Volume:12 | Issue:1 Page(s): 1-11

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Title

Optimization of Culture Media Parameters of Saccharomyces Cerevisiae PTCC 5209 for Maximized Invertase Enzyme Production

Author(s)

Nejatinejad Melika | Fooladi Jamshid | Issue Writer Certificate

Keywords

Invertase

Optimization

Saccharomyces cerevisiae PTCC 5209

Yeast fermentation

Abstract

Background and Objective: Invertase enzyme or D-fructofuranosidfructohydrolase EC (3. 2. 1. 26) is a member of the hydrolase family and responsible for the decomposition of sucrose into fructose and glucose. In recent years, extensive research has been carried out to increase the industrial production of invertase enzyme. Material and Methods: This study focused on maximizing invertase production in Saccharomyces cerevisiae by optimizing culture media conditions. Elements of the culture media were investigated using monofunctional optimization method. Moreover, basic salt culture media, containing compounds such as Na₂HPO₄, K₂HPO₄, MgSO₄ and CaCl₂, were used. Then, growth curve of the yeast was plotted and results showed that the highest growth rate occurred within 38 h and the strongest enzyme activity occurred within 18 h. Optimizing the culture conditions showed that yeast provided the most activity with 1% sucrose as a carbon source, urea and 0. 5% meat peptone as nitrogen sources, pH 5, 30 °C and shaking speed of 150 rpm. In this research, 3-l fermentor was used to assess yeast growth and enzyme activity at a larger scale. Results and Conclusion: Results of this study showed that the highest OD value was included at 48 h and the highest enzyme activity was recorded at 28 and 96 h. The difference between the time of maximum growth and peak enzyme activity indicated the need of careful control of fermentation time to prevent unnecessary biomass accumulation. Therefore, further research in the field of advanced fermentation and optimization of yeast strains can help researchers achieve the highest secretion and enzyme activity.

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