EVALUATION OF THE DIFFERENTIATION PROCESS OF HUMAN ADIPOSE TISSUEDERIVED MESENCHYMAL STEM CELLS TO SKELETAL MUSCLE-LIKE CELLS: AN IN VITRO STUDY

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

NAMJOO GERMI Z.; HEYDARI M.H.; NOROUZIAN M.; MASTARI FARAHANI M.; ALTARIHI T.; PIRYAEI M.; BAYATI V.; HEYDARI M.H.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of North Khorasan University of Medical Sciences Year:2013 | Volume:5 | Issue:1 Page(s): 147-158

Download Full-Text

Persian Verion

View:

1,474

Download:

Cites:

Information Journal Paper

Title

EVALUATION OF THE DIFFERENTIATION PROCESS OF HUMAN ADIPOSE TISSUEDERIVED MESENCHYMAL STEM CELLS TO SKELETAL MUSCLE-LIKE CELLS: AN IN VITRO STUDY

Author(s)

Keywords

ADIPOSE TISSUEQ2

MESENCHYMAL STEM CELLSQ2

DIFFERENTIATIONQ2

SKELETAL MUSCLE CELLQ2

Abstract

Background Objectives: This study was carried out to evaluate the development of skeletal muscle-like cells derived from human ADIPOSE TISSUE MESENCHYMAL STEM CELLS induced by 5-azacytidine [5- aza] under in vitro condition.Materials and methods: Human ADIPOSE TISSUE MESENCHYMAL STEM CELLS [ADSCs] were purified. These cells were cultured in osteogenic or adipogenic induction medium to induce osteogenic and adipogenic DIFFERENTIATION. On the other hand, the skeletal myogenic DIFFERENTIATION potential of these cells was investigated using 3mmol 5-azacytidine [5-aza] treatment for 24 hours. Then the culture was washed out by PBS and were put in the culture medium free of 5-aza for another 4 weeks until sampling. RT-PCR assay was performed to detect the expression of specific skeletal muscle genes including Myogenin, Myh, alpha-actin, tropomyosin and myosin at 1-4 weeks after the first induction.Results: The adherent ADIPOSE TISSUE-derived MESENCHYMAL STEM CELLS exhibited a significant proliferative capacity and also showed osteogenic and adipogenic DIFFERENTIATION as seen in previous studies. The expression of four SKELETAL MUSCLE CELLs genes was detected 1st to 4th week after induction in a time-dependent manner.There was a continuous increase in expression of Myogenin gene from the 1st to 4th week, whereas that of the Myh was higher after two weeks and lower after 4 weeks in comparison to the other weeks.The levels of mRNA of alpha-actin and Myosin expression were at the highest level in the fourth week. Expression of tropomyosin gene was not significant in the samples.Conclusion: The current study indicated that stimulating human ADIPOSE TISSUE-derived MESENCHYMAL STEM CELLS by 5-aza with the concentration of 3mmol under in vitro condition can lead to differentiating skeletal muscle-like cells. Furthermore prolonged culture duration may lead to more differentiated cells.

Cites

No record.

References

No record.

Cite

APA: Copy

NAMJOO GERMI, Z., HEYDARI, M.H., NOROUZIAN, M., MASTARI FARAHANI, M., ALTARIHI, T., PIRYAEI, M., BAYATI, V., & HEYDARI, M.H.. (2013). EVALUATION OF THE DIFFERENTIATION PROCESS OF HUMAN ADIPOSE TISSUEDERIVED MESENCHYMAL STEM CELLS TO SKELETAL MUSCLE-LIKE CELLS: AN IN VITRO STUDY. JOURNAL OF NORTH KHORASAN UNIVERSITY OF MEDICAL SCIENCES, 5(1), 147-158. SID. https://sid.ir/paper/187097/en

Vancouver: Copy

NAMJOO GERMI Z., HEYDARI M.H., NOROUZIAN M., MASTARI FARAHANI M., ALTARIHI T., PIRYAEI M., BAYATI V., HEYDARI M.H.. EVALUATION OF THE DIFFERENTIATION PROCESS OF HUMAN ADIPOSE TISSUEDERIVED MESENCHYMAL STEM CELLS TO SKELETAL MUSCLE-LIKE CELLS: AN IN VITRO STUDY. JOURNAL OF NORTH KHORASAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2013;5(1):147-158. Available from: https://sid.ir/paper/187097/en

IEEE: Copy

Z. NAMJOO GERMI, M.H. HEYDARI, M. NOROUZIAN, M. MASTARI FARAHANI, T. ALTARIHI, M. PIRYAEI, V. BAYATI, and M.H. HEYDARI, “EVALUATION OF THE DIFFERENTIATION PROCESS OF HUMAN ADIPOSE TISSUEDERIVED MESENCHYMAL STEM CELLS TO SKELETAL MUSCLE-LIKE CELLS: AN IN VITRO STUDY,” JOURNAL OF NORTH KHORASAN UNIVERSITY OF MEDICAL SCIENCES, vol. 5, no. 1, pp. 147–158, 2013, [Online]. Available: https://sid.ir/paper/187097/en

Related Journal Papers