CHARACTERIZATION OF RAT ESOPHAGUS DECELLULARIZED SCAFFOLD INDUCTIVE EFFECT ON ARRANGEMENT OF HUMAN ADIPOSE DERIVED MESENCHYMAL STEM CELLS

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NADERI S.; AKBARZADEH M.; MAHDAVI SHAHRI N.; MATIN M.M.; FEREIDONI M.; HAGHIGHITALAB A.

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Journal: Journal of North Khorasan University of Medical Sciences Year:2013 | Volume:5 | Issue:1 Page(s): 139-146

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Title

CHARACTERIZATION OF RAT ESOPHAGUS DECELLULARIZED SCAFFOLD INDUCTIVE EFFECT ON ARRANGEMENT OF HUMAN ADIPOSE DERIVED MESENCHYMAL STEM CELLS

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Keywords

ESOPHAGUSQ2

3D ARRANGEMENTQ2

MESENCHYMAL STEM CELLSQ2

TISSUE ENGINEERINGQ2

Abstract

Background & objectives: TISSUE ENGINEERING techniques try to produce artificial tissues with properties like normal tissues.Regardless of progress in TISSUE ENGINEERING techniques especially in design and synthesis of suitable scaffolds, loading of cells into scaffolds is a serious obstacle in obtaining proper results in TISSUE ENGINEERING. Aim of this study was to prepare scaffolds from decellularized ESOPHAGUS tissue with dimensions like normal tissue to study arrangement capability of cultured human adipose derived MESENCHYMAL STEM CELLS (AD-MSCs).Methods: After removing the ESOPHAGUS, physical and chemical decellularization methods including snap freeze-thaw and treatment with Triton X-100 and sodium dodecyl sulfate (SDS), as detergents, were performed. The scaffolds, were then cultivated with human AD-MSCs at a density of 5´105 cells /cm2.Microscopic sections were prepared from the scaffolds before and after 2 weeks of culture with cells and were studied.Results: Histological studies showed that proper migration and extension of MESENCHYMAL STEM CELLS in ESOPHAGUS matrix led to arrangement of these cells in different layers of the prepared scaffold.Conclusion: considering layers structure of ESOPHAGUS matrix, prepared scaffold might be a suitable scaffold for studying cell behaviors and preliminary studies in ESOPHAGUS TISSUE ENGINEERING.

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APA: Copy

NADERI, S., AKBARZADEH, M., MAHDAVI SHAHRI, N., MATIN, M.M., FEREIDONI, M., & HAGHIGHITALAB, A.. (2013). CHARACTERIZATION OF RAT ESOPHAGUS DECELLULARIZED SCAFFOLD INDUCTIVE EFFECT ON ARRANGEMENT OF HUMAN ADIPOSE DERIVED MESENCHYMAL STEM CELLS. JOURNAL OF NORTH KHORASAN UNIVERSITY OF MEDICAL SCIENCES, 5(1), 139-146. SID. https://sid.ir/paper/187102/en

Vancouver: Copy

NADERI S., AKBARZADEH M., MAHDAVI SHAHRI N., MATIN M.M., FEREIDONI M., HAGHIGHITALAB A.. CHARACTERIZATION OF RAT ESOPHAGUS DECELLULARIZED SCAFFOLD INDUCTIVE EFFECT ON ARRANGEMENT OF HUMAN ADIPOSE DERIVED MESENCHYMAL STEM CELLS. JOURNAL OF NORTH KHORASAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2013;5(1):139-146. Available from: https://sid.ir/paper/187102/en

IEEE: Copy

S. NADERI, M. AKBARZADEH, N. MAHDAVI SHAHRI, M.M. MATIN, M. FEREIDONI, and A. HAGHIGHITALAB, “CHARACTERIZATION OF RAT ESOPHAGUS DECELLULARIZED SCAFFOLD INDUCTIVE EFFECT ON ARRANGEMENT OF HUMAN ADIPOSE DERIVED MESENCHYMAL STEM CELLS,” JOURNAL OF NORTH KHORASAN UNIVERSITY OF MEDICAL SCIENCES, vol. 5, no. 1, pp. 139–146, 2013, [Online]. Available: https://sid.ir/paper/187102/en

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