Investigation of mutations in hotspot regions of ERG11 gene in
fluconazole-resistant isolates of Candida albicans
in the west of Mazandaran

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Majdi Masoumeh; KHAZAEI KOOHPAR ZEINAB; NASROLLAHI OMRAN AYATOLLAH

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Journal: JOURNAL OF MICROBIAL WORLD Year:2018 | Volume:11 | Issue:3 (36) Page(s): 258-268

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Information Journal Paper

Title

Investigation of mutations in hotspot regions of ERG11 gene in fluconazole-resistant isolates of Candida albicans in the west of Mazandaran

Author(s)

Majdi Masoumeh | KHAZAEI KOOHPAR ZEINAB | NASROLLAHI OMRAN AYATOLLAH | Issue Writer Certificate

Keywords

Candida albicansQ1

ERG11Q1

FluconazoleQ1

V488I mutationQ1

D504V mutationQ1

Abstract

Background & Objectives: Nowadays, widespread use of Fluconazole has resulted in resistance in Candida albicans strains. The conformational changes of ERG11p due to mutations in the ERG11 gene is one of the mechanisms resulting in azole resistance. The aim of our study was to investigate ERG11 gene mutations in Fluconazole-resistant isolates of C. albicans in the west of Mazandaran province. Materials & Methods: In this descriptive cross-sectional study, clinical specimens were obtained from vaginal mucosa of 120 women in hospitals of the west of Mazandaran province. C. albicans isolates were identified by standard methods such as germ tubes and CHROMEagar medium culture. The Fluconazole resistance and susceptibility of the isolates were evaluated by Kirby Bauer and broth micro-dilution methods. Then, ERG11 gene mutations in resistant isolates were determined by PCR-sequencing method as compared with PTCC5027 (ATCC10231) reference strain. Results: Out of 45 C. albicans isolates, 40 isolates were resistant and 5 isolates were susceptible to luconazole. The MIC o luconazole a etermine a ≥ 6 μ g/ml. PCR-sequencing analysis revealed that 18 Fluconazole-resistant isolates have six missense mutations (Y257H, E266D, V404I, D421N, V488I, and D504V) in the ERG11 gene. Conclusion: The identified mutations in this study may play role in developing Fluconazole resistance in C. albicans isolates in the west of Mazandaran province by decreasing Fluconazole affinity to ERG11p.

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APA: Copy

Majdi, Masoumeh, KHAZAEI KOOHPAR, ZEINAB, & NASROLLAHI OMRAN, AYATOLLAH. (2018). Investigation of mutations in hotspot regions of ERG11 gene in fluconazole-resistant isolates of Candida albicans in the west of Mazandaran. JOURNAL OF MICROBIAL WORLD, 11(3 (36) ), 258-268. SID. https://sid.ir/paper/189215/en

Vancouver: Copy

Majdi Masoumeh, KHAZAEI KOOHPAR ZEINAB, NASROLLAHI OMRAN AYATOLLAH. Investigation of mutations in hotspot regions of ERG11 gene in fluconazole-resistant isolates of Candida albicans in the west of Mazandaran. JOURNAL OF MICROBIAL WORLD[Internet]. 2018;11(3 (36) ):258-268. Available from: https://sid.ir/paper/189215/en

IEEE: Copy

Masoumeh Majdi, ZEINAB KHAZAEI KOOHPAR, and AYATOLLAH NASROLLAHI OMRAN, “Investigation of mutations in hotspot regions of ERG11 gene in fluconazole-resistant isolates of Candida albicans in the west of Mazandaran,” JOURNAL OF MICROBIAL WORLD, vol. 11, no. 3 (36) , pp. 258–268, 2018, [Online]. Available: https://sid.ir/paper/189215/en

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