IDENTIFICATION OF FLUCONAZOLE RESISTANCE GENE ERG11 IN CLINICAL CANDIDA ALBICANS SAMPLES ISOLATED FROM HIV-INFECTED PATIENTS BY REVERSE POLYMERASE CHAIN REACTION (RT-PCR)

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FARAHBAKHSH EHSAN; YADEGARI MOHAMMAD HOSSEIN; RAJABI BAZL MASOUMEH; TAGHIZADEH ARMAKI MOJTABA; KATIRAEE FARZAD

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Journal: DANESHVAR MEDICINE Year:2012 | Volume:19 | Issue:96 Page(s): 19-28

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Title

IDENTIFICATION OF FLUCONAZOLE RESISTANCE GENE ERG11 IN CLINICAL CANDIDA ALBICANS SAMPLES ISOLATED FROM HIV-INFECTED PATIENTS BY REVERSE POLYMERASE CHAIN REACTION (RT-PCR)

Author(s)

Keywords

CANDIDA ALBICANSQ2

AIDSQ3

ERG11 GENEQ2

RT-PCR METHODQ2

Abstract

Background and Objective: Nowadays opportunistic fungi especially C.albicans are the most common cause of life-threating infections in immunodeficiency patients. Increasing Azole-resistant strains of C.albicans are a main problem in human immunodeficiency virus-infected patients. The aim of this study was the evaluation of ERG11 GENE in C.albicans Azole resistant strains that were isolated from AIDS patients with oropharyngeal candidiasis by RT-PCR METHOD.Materials and Methods: The present experimental study was conducted at Tarbiat Modares University during the years 2009-2011. C. albicans isolates from HIV-infected patients were identified by standard procedures including germ tube formation, clamydoconidia and color of colonies on CHROM agar. At first, susceptibility of C. albicans isolates was assessed by disk diffusion agar technique.Then, overexpression of fluconazole resistance gene ERG11 using RT-PCR was compared with control samples and gene expression levels were determined by UVItec software.Results: The results of drug sensitivity for 66 C. albicans isolated from AIDS patients showed that 62.6% were susceptible, 8.6% were susceptible-dose dependent (SDD) and 28.7% were resistant.Product evaluation of RT-PCR showed that 9% of patients have increased expression of ERG11gene resistance.Conclusion: Oral candidiasis is a frequent complication among Iranian HIV individuals. The results of this study showed the high percentage of resistant strains among samples of C. albicans. However, the use of phenotypic methods like disk diffusion agar which has lower costs together with genotypic methods like RT-PCR which provide the possibility of studying the mechanism of drug resistance and genes involved is highly recommended.

Cites

CORRELATION BETWEEN ERG11 GENE MUTATIONS AND FLUCONAZOLE RESISTANCE IN CANDIDA ALBICANS STRAINS ISOLATED FROM RASHT IN YEARS 2015-2016

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APA: Copy

FARAHBAKHSH, EHSAN, YADEGARI, MOHAMMAD HOSSEIN, RAJABI BAZL, MASOUMEH, TAGHIZADEH ARMAKI, MOJTABA, & KATIRAEE, FARZAD. (2012). IDENTIFICATION OF FLUCONAZOLE RESISTANCE GENE ERG11 IN CLINICAL CANDIDA ALBICANS SAMPLES ISOLATED FROM HIV-INFECTED PATIENTS BY REVERSE POLYMERASE CHAIN REACTION (RT-PCR). DANESHVAR MEDICINE, 19(96), 19-28. SID. https://sid.ir/paper/30386/en

Vancouver: Copy

FARAHBAKHSH EHSAN, YADEGARI MOHAMMAD HOSSEIN, RAJABI BAZL MASOUMEH, TAGHIZADEH ARMAKI MOJTABA, KATIRAEE FARZAD. IDENTIFICATION OF FLUCONAZOLE RESISTANCE GENE ERG11 IN CLINICAL CANDIDA ALBICANS SAMPLES ISOLATED FROM HIV-INFECTED PATIENTS BY REVERSE POLYMERASE CHAIN REACTION (RT-PCR). DANESHVAR MEDICINE[Internet]. 2012;19(96):19-28. Available from: https://sid.ir/paper/30386/en

IEEE: Copy

EHSAN FARAHBAKHSH, MOHAMMAD HOSSEIN YADEGARI, MASOUMEH RAJABI BAZL, MOJTABA TAGHIZADEH ARMAKI, and FARZAD KATIRAEE, “IDENTIFICATION OF FLUCONAZOLE RESISTANCE GENE ERG11 IN CLINICAL CANDIDA ALBICANS SAMPLES ISOLATED FROM HIV-INFECTED PATIENTS BY REVERSE POLYMERASE CHAIN REACTION (RT-PCR),” DANESHVAR MEDICINE, vol. 19, no. 96, pp. 19–28, 2012, [Online]. Available: https://sid.ir/paper/30386/en

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