CORRELATION BETWEEN ERG11 GENE MUTATIONS AND FLUCONAZOLE RESISTANCE IN CANDIDA ALBICANS STRAINS ISOLATED FROM RASHT IN YEARS 2015-2016

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BALABANDI SAEEDEH; KHAZAEI KOOHPAR ZEINAB; RANJI NAJMEH

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Journal: Journal of Arak University Medical Sciences Year:2017 | Volume:20 | Issue:7 (124) Page(s): 13-22

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Title

CORRELATION BETWEEN ERG11 GENE MUTATIONS AND FLUCONAZOLE RESISTANCE IN CANDIDA ALBICANS STRAINS ISOLATED FROM RASHT IN YEARS 2015-2016

Author(s)

BALABANDI SAEEDEH | KHAZAEI KOOHPAR ZEINAB | RANJI NAJMEH | Issue Writer Certificate

Keywords

CANDIDA ALBICANSQ2

ERG11Q1

FLUCONAZOLEQ1

MICQ1

MUTATIONQ2

Abstract

Background: CANDIDA ALBICANS as an opportunistic fungal pathogen in human causes candidiasis. The widespread use of azoles has led to the increase of azole resistance in CANDIDA ALBICANS isolates. MUTATION in theERG11 gene is one of several azole resistance reasons in CANDIDA ALBICANS. The aim of this study was to findERG11 gene MUTATIONs in FLUCONAZOLE resistant isolates in Rasht.Materials and methods: CANDIDA ALBICANSisolates were identified by standard identification methods such as germ tubes. The FLUCONAZOLE resistance and susceptibility of the isolates was evaluated by Disc diffusion and MIC methods. For MUTATION determining, ERG11 gene was amplified by PCR and then sequenced in clinical isolates.Results: From 23 isolates of CANDIDA ALBICANS, 20 isolates were FLUCONAZOLE resistant. The MIC of FLUCONAZOLE in these isolates was determined between 128 to 2048μg/ml. Also, sequencing analysis showed that 10 FLUCONAZOLE resistant isolates had two missense MUTATIONs (D116E and E266D) inERG11 gene.Conclusion: In this study, resistance to high concentration of FLUCONAZOLE shows that different mechanisms simultaneously implicated in developing azoles resistance in the isolates. Association of ERG11gene MUTATION and deregulation of other genes can be led to resistance to high FLUCONAZOLE concentration in this study.

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APA: Copy

BALABANDI, SAEEDEH, KHAZAEI KOOHPAR, ZEINAB, & RANJI, NAJMEH. (2017). CORRELATION BETWEEN ERG11 GENE MUTATIONS AND FLUCONAZOLE RESISTANCE IN CANDIDA ALBICANS STRAINS ISOLATED FROM RASHT IN YEARS 2015-2016. ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ), 20(7 (124)), 13-22. SID. https://sid.ir/paper/69712/en

Vancouver: Copy

BALABANDI SAEEDEH, KHAZAEI KOOHPAR ZEINAB, RANJI NAJMEH. CORRELATION BETWEEN ERG11 GENE MUTATIONS AND FLUCONAZOLE RESISTANCE IN CANDIDA ALBICANS STRAINS ISOLATED FROM RASHT IN YEARS 2015-2016. ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ)[Internet]. 2017;20(7 (124)):13-22. Available from: https://sid.ir/paper/69712/en

IEEE: Copy

SAEEDEH BALABANDI, ZEINAB KHAZAEI KOOHPAR, and NAJMEH RANJI, “CORRELATION BETWEEN ERG11 GENE MUTATIONS AND FLUCONAZOLE RESISTANCE IN CANDIDA ALBICANS STRAINS ISOLATED FROM RASHT IN YEARS 2015-2016,” ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ), vol. 20, no. 7 (124), pp. 13–22, 2017, [Online]. Available: https://sid.ir/paper/69712/en

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