COMPARISON OF BACTERIAL CULTURE, ELISA AND PCR TECHNIQUES FOR DETECTION OF SALMONELLA IN POULTRY MEAT SAMPLES COLLECTED FROM TEHRAN

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HOSSEINPOUR MOHSEN; SABOKBAR AZAR; BAKHTIARI AMIR; PARSA SHAHNAZ

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Journal: JOURNAL OF MICROBIAL WORLD Year:2013 | Volume:6 | Issue:1 (14) Page(s): 62-72

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Information Journal Paper

Title

COMPARISON OF BACTERIAL CULTURE, ELISA AND PCR TECHNIQUES FOR DETECTION OF SALMONELLA IN POULTRY MEAT SAMPLES COLLECTED FROM TEHRAN

Author(s)

HOSSEINPOUR MOHSEN | SABOKBAR AZAR | BAKHTIARI AMIR | PARSA SHAHNAZ | Issue Writer Certificate

Keywords

SALMONELLAQ2

ELISAQ2

POLYMERASE CHAIN REACTIONQ2

INVAQ2

Abstract

Background and Objectives: SALMONELLA is one of the most important causative agents of food poisoning and gastroenteritis in humans. Using the standard culture methods for detection and isolation of SALMONELLA and also, the evaluation of bacterial characteristics by biochemical and serological tests, need to take 4 to 7 days, accuracy and skill of the lab staffs and quality of cultured media. Current study was carried out to assess PCR method for rapid detection of SALMONELLA in poultry meat samples and to compare its quality with two currently available standard culture and ELISA methods.Materials and Methods: This cross-sectional study was carried out on 60 samples of poultry meat collected from food stores of Tehran. The frequency of SALMONELLA was evaluated by using three microbiological methods culturing, ELISA (Kit SAL-VIA96; TECRA) and PCR (amplification of INVA gene). Also accuracy, sensitivity and specificity of the methods were compared with each other.Results: In total of samples, the incidence of SALMONELLA were 55%, 47% and 65%, by using culture, ELISA and PCR methods, respectively. Our findings showed that PCR based detection of SALMONELLA using INVA primers and only with a twenty hour pre-enrichment of samples in BPW is capable identifying SALMONELLA with high sensitivity and specificity (both 100%) in food samples in 24 to 30 hours.Conclusion: This study showed that PCR method has appropriate efficiency and sensitivity for rapid screening of SALMONELLA. Therefore, this technique is recommended to evaluate of SALMONELLA in food samples.

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APA: Copy

HOSSEINPOUR, MOHSEN, SABOKBAR, AZAR, BAKHTIARI, AMIR, & PARSA, SHAHNAZ. (2013). COMPARISON OF BACTERIAL CULTURE, ELISA AND PCR TECHNIQUES FOR DETECTION OF SALMONELLA IN POULTRY MEAT SAMPLES COLLECTED FROM TEHRAN. JOURNAL OF MICROBIAL WORLD, 6(1 (14)), 62-72. SID. https://sid.ir/paper/189254/en

Vancouver: Copy

HOSSEINPOUR MOHSEN, SABOKBAR AZAR, BAKHTIARI AMIR, PARSA SHAHNAZ. COMPARISON OF BACTERIAL CULTURE, ELISA AND PCR TECHNIQUES FOR DETECTION OF SALMONELLA IN POULTRY MEAT SAMPLES COLLECTED FROM TEHRAN. JOURNAL OF MICROBIAL WORLD[Internet]. 2013;6(1 (14)):62-72. Available from: https://sid.ir/paper/189254/en

IEEE: Copy

MOHSEN HOSSEINPOUR, AZAR SABOKBAR, AMIR BAKHTIARI, and SHAHNAZ PARSA, “COMPARISON OF BACTERIAL CULTURE, ELISA AND PCR TECHNIQUES FOR DETECTION OF SALMONELLA IN POULTRY MEAT SAMPLES COLLECTED FROM TEHRAN,” JOURNAL OF MICROBIAL WORLD, vol. 6, no. 1 (14), pp. 62–72, 2013, [Online]. Available: https://sid.ir/paper/189254/en

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