ISOLATION AND IDENTIFICATION OF PRODUCING SIALIDASE ENZYME BACTERIA ISOLATED FROM NORMAL MICROFLORA OF MOUTH

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HOSSEINI FARZANEH; RAZAVIPOUR ROUYA; EBRAHIMI ELMIRA

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Journal: JOURNAL OF MICROBIAL WORLD Year:2012 | Volume:5 | Issue:1-2 (12) Page(s): 30-38

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Information Journal Paper

Title

ISOLATION AND IDENTIFICATION OF PRODUCING SIALIDASE ENZYME BACTERIA ISOLATED FROM NORMAL MICROFLORA OF MOUTH

Author(s)

HOSSEINI FARZANEH | RAZAVIPOUR ROUYA | EBRAHIMI ELMIRA | Issue Writer Certificate

Keywords

SIALIDASE ENZYMEQ2

GANGLIOSIDESQ2

MUNQ2

Abstract

Background and Objectives: Production of Sialidase by oral microbial flora leads to degradation of glycoproteins and GANGLIOSIDES on the surfaces of the cells, leading to cell physiologic changes and increase oral diseases. This enzyme can be isolated from various microorganisms including bacteria, protozoa and etc. This study was aimed to isolate sialidase producing bacteria from normal flora of mouth.Material and Methods: This cross-sectional descriptive study conducted on 94 mouth swab samples of individuals who visited by dentists in the Shahryar dental clinic. Isolation and identification of bacteria was performed by specific assays and culture media. Sialidase producing bacteria were identified by sensitive fluorometric assay and using 2ʹ-(4-methylumbelliferyl) a-D-N acetylneuraminic acid as substrate. Activity and stability of enzyme was studied in different condition of pH and temperature.Results: In this study, isolated bacteria belonged to Streptococcus, Bacterioedes, Fusobacterium, Capnocytophaga and Actinobacilus. Isolated Streptococcus strains were showed 98% homology with S. pneumonia, S. mutans, S. sanguis, S. salivarius and S. oralis species. The higher enzyme activity was observed in Streptococcus and Bacterioedes in optimum pH 6 and 35oC. Also, enzyme production was accomplished parallel with growth of bacteria.Conclusion: Mouth normal flora of healthy individuals contain several sialidase producing bacteria which could use as cheap and available source of this enzyme for medical and industrial application.

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APA: Copy

HOSSEINI, FARZANEH, RAZAVIPOUR, ROUYA, & EBRAHIMI, ELMIRA. (2012). ISOLATION AND IDENTIFICATION OF PRODUCING SIALIDASE ENZYME BACTERIA ISOLATED FROM NORMAL MICROFLORA OF MOUTH. JOURNAL OF MICROBIAL WORLD, 5(1-2 (12)), 30-38. SID. https://sid.ir/paper/189275/en

Vancouver: Copy

HOSSEINI FARZANEH, RAZAVIPOUR ROUYA, EBRAHIMI ELMIRA. ISOLATION AND IDENTIFICATION OF PRODUCING SIALIDASE ENZYME BACTERIA ISOLATED FROM NORMAL MICROFLORA OF MOUTH. JOURNAL OF MICROBIAL WORLD[Internet]. 2012;5(1-2 (12)):30-38. Available from: https://sid.ir/paper/189275/en

IEEE: Copy

FARZANEH HOSSEINI, ROUYA RAZAVIPOUR, and ELMIRA EBRAHIMI, “ISOLATION AND IDENTIFICATION OF PRODUCING SIALIDASE ENZYME BACTERIA ISOLATED FROM NORMAL MICROFLORA OF MOUTH,” JOURNAL OF MICROBIAL WORLD, vol. 5, no. 1-2 (12), pp. 30–38, 2012, [Online]. Available: https://sid.ir/paper/189275/en

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