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Information Journal Paper

Title

ISOLATION AND MOLECULAR IDENTIFICATION OF RHODOBACTER SPHAEROIDES FROM ANAEROBIC LAGOON OF WASTEWATER TREATMENT SYSTEM

Pages

  23-29

Keywords

H2S OXIDATIONQ2

Abstract

 Background and Objectives: RHODOBACTER is a gram negative rod shaped purple phototrophic bacteria capable of oxidizing H2S to S and thus capable of elimination of H2S from nature. This project aimed to isolate and identify RHODOBACTER sphaeroides from nature.Material and Methods: The water samples were collected from anaerobic lagoon wastewater of Mahdi-shahr wastewater treatment plant. The samples then were cultured on Pfennig'S Medium I. After growing the bacteria, the colonies were investigated based on 16S RRNA gene amplification. Finally, the PCR products were sequenced and were investigated by BLAST to determine the phylogenetic of the isolated bacteria.Results: Macroscopic studies on the isolated red colonies showed gram negative bacilli. Based on the 16S RRNA sequence studies, this bacterium belonged RHODOBACTER and had 99% similarity to RHODOBACTER sphaeroides.Conclusion: Fist time in Iran, RHODOBACTER sphaeroides was isolated. Also, this study could isolate a new strain of RHODOBACTER sphaeroides was isolated from waste water and was registered in gene bank with IRSMM1 name and JX262384 acceptation number.

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  • Cite

    APA: Copy

    MOGHBELI, MAJID, & SHAFAATI, MOHAMMAD REZA. (2012). ISOLATION AND MOLECULAR IDENTIFICATION OF RHODOBACTER SPHAEROIDES FROM ANAEROBIC LAGOON OF WASTEWATER TREATMENT SYSTEM. JOURNAL OF MICROBIAL WORLD, 5(1-2 (12)), 23-29. SID. https://sid.ir/paper/189277/en

    Vancouver: Copy

    MOGHBELI MAJID, SHAFAATI MOHAMMAD REZA. ISOLATION AND MOLECULAR IDENTIFICATION OF RHODOBACTER SPHAEROIDES FROM ANAEROBIC LAGOON OF WASTEWATER TREATMENT SYSTEM. JOURNAL OF MICROBIAL WORLD[Internet]. 2012;5(1-2 (12)):23-29. Available from: https://sid.ir/paper/189277/en

    IEEE: Copy

    MAJID MOGHBELI, and MOHAMMAD REZA SHAFAATI, “ISOLATION AND MOLECULAR IDENTIFICATION OF RHODOBACTER SPHAEROIDES FROM ANAEROBIC LAGOON OF WASTEWATER TREATMENT SYSTEM,” JOURNAL OF MICROBIAL WORLD, vol. 5, no. 1-2 (12), pp. 23–29, 2012, [Online]. Available: https://sid.ir/paper/189277/en

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