PHENOTYPIC AND GENETICALLY EVALUATION OF THE PREVALENCE OF EFFLUX PUMPS AND ANTIBIOTIC RESISTANCE IN CLINICAL ISOLATES OF PSEUDOMONAS AERUGINOSA AMONG BURNED PATIENTS ADMITTED TO GHOTBODIN SHIRAZI HOSPITAL

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

DASHTIZADEH YAHYA; MOATTARI AFAGH; GORZIN ALI AKBAR

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF MICROBIAL WORLD Year:2014 | Volume:7 | Issue:2 (19) Page(s): 118-127

Download Full-Text

Persian Verion

View:

2,175

Download:

Cites:

Information Journal Paper

Title

PHENOTYPIC AND GENETICALLY EVALUATION OF THE PREVALENCE OF EFFLUX PUMPS AND ANTIBIOTIC RESISTANCE IN CLINICAL ISOLATES OF PSEUDOMONAS AERUGINOSA AMONG BURNED PATIENTS ADMITTED TO GHOTBODIN SHIRAZI HOSPITAL

Author(s)

DASHTIZADEH YAHYA | MOATTARI AFAGH | GORZIN ALI AKBAR | Issue Writer Certificate

Keywords

BURNED PATIENTSQ2

PSEUDOMONAS AERUGINOSAQ2

EFFLUX PUMPSQ2

Abstract

Background & Objectives: PSEUDOMONAS AERUGINOSA is an important cause of nosocomial infections in burnt patients. Antibiotic resistance through various mechanisms is one of challenges for treatment of these patients. The mex EFFLUX PUMPS play a vital role in the development of multiple resistances to antimicrobial drugs. This study aimed to analyze the prevalence of genes responsible for EFFLUX PUMPS mexA-B-oprM and to investigate their phenotypes in the isolates.Materials & Methods: In this cross-sectional study, 250 swabs were obtained from wounds of the patients suffering of burns levels 2 and 3 who admitted in Ghotbodin Shirazi hospital, Shiraz. Presence of P. aeruginosa isolates were confirmed by biochemical tests and PCR. Drug susceptibility, the phenotypic activity of EFFLUX PUMPS and the presence of mex A, mex B genes were determined using disk diffusion method, cartwheel method and PCR methods, respectively.Results: In this study, 26.40% (66 cases) of patients with burn wounds were infected with P. aeruginosa. These bacteria were resistant to all antibiotics tested except for colistin. Totally, 66.66% of the isolates (44 cases) had an efflux pump, among them 42.92% and 87.87% of the isolates carried mexA and mexB genes, respectively.Conclusion: Our finding showed that genotypic method is very accurate and reliable than phenotypic methods for detection of EFFLUX PUMPS in clinical isolates of P. aeruginosa. Due to presence of the efflux pump genes in more than 90% of the P. aeruginosa isolates, analysis of the presence of these genes is very important for suggestion of an effective treatment model for the patients with bacterial infection.

Cites

FREQUENCY OF PLASMID-MEDIATED QUINOLONE RESISTANCE GENES AMONG CLINICAL ISOLATES OF PSEUDOMONAS AERUGINOSA IN KERMAN, IRAN

References

No record.

Cite

APA: Copy

DASHTIZADEH, YAHYA, MOATTARI, AFAGH, & GORZIN, ALI AKBAR. (2014). PHENOTYPIC AND GENETICALLY EVALUATION OF THE PREVALENCE OF EFFLUX PUMPS AND ANTIBIOTIC RESISTANCE IN CLINICAL ISOLATES OF PSEUDOMONAS AERUGINOSA AMONG BURNED PATIENTS ADMITTED TO GHOTBODIN SHIRAZI HOSPITAL. JOURNAL OF MICROBIAL WORLD, 7(2 (19)), 118-127. SID. https://sid.ir/paper/189385/en

Vancouver: Copy

DASHTIZADEH YAHYA, MOATTARI AFAGH, GORZIN ALI AKBAR. PHENOTYPIC AND GENETICALLY EVALUATION OF THE PREVALENCE OF EFFLUX PUMPS AND ANTIBIOTIC RESISTANCE IN CLINICAL ISOLATES OF PSEUDOMONAS AERUGINOSA AMONG BURNED PATIENTS ADMITTED TO GHOTBODIN SHIRAZI HOSPITAL. JOURNAL OF MICROBIAL WORLD[Internet]. 2014;7(2 (19)):118-127. Available from: https://sid.ir/paper/189385/en

IEEE: Copy

YAHYA DASHTIZADEH, AFAGH MOATTARI, and ALI AKBAR GORZIN, “PHENOTYPIC AND GENETICALLY EVALUATION OF THE PREVALENCE OF EFFLUX PUMPS AND ANTIBIOTIC RESISTANCE IN CLINICAL ISOLATES OF PSEUDOMONAS AERUGINOSA AMONG BURNED PATIENTS ADMITTED TO GHOTBODIN SHIRAZI HOSPITAL,” JOURNAL OF MICROBIAL WORLD, vol. 7, no. 2 (19), pp. 118–127, 2014, [Online]. Available: https://sid.ir/paper/189385/en

Related Journal Papers