ASSESSMENT OF CXCL12 GENE EXPRESSION IN HUMAN LUNG ADENOCARCINOMA CELL LINE A549 BY REAL TIME PCR METHOD

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MAHBOBI R.; FALLAH MEHRABADI J.; ALI ASGARI E.

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Journal: JOURNAL OF ANIMAL PHYSIOLOGY AND DEVELOPMENT (QUARTERLY JOURNAL OF BIOLOGICAL SCIENCES) Year:2016 | Volume:9 | Issue:3 (34) Page(s): 39-45

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Title

ASSESSMENT OF CXCL12 GENE EXPRESSION IN HUMAN LUNG ADENOCARCINOMA CELL LINE A549 BY REAL TIME PCR METHOD

Author(s)

MAHBOBI R. | FALLAH MEHRABADI J. | ALI ASGARI E. | Issue Writer Certificate

Keywords

CXCL12Q1

A549 CELL LINEQ1

REAL TIME PCRQ1

LUNG CANCERQ2

Abstract

Inroduction & Objective: LUNG CANCER is the most common malignancy and the leading cause of cancer related mortality among women and men worldwide, in such a way that the 5-year survival of patients is about 15%. According to the invasive role of CXCL12, challenges in various studies about expression and non-expression of CXCL12 and the presence of metastasis in early stages of lung adenocarcinoma, the A549 CELL LINE is selected in order to investigate CXCL12 gene expression in it.Material and Methods: In this study A549 CELL LINE was cultured in a DMEM medium contain 10% fetal serum bovine and 1% penicillin- streptomycin. RNA extraction was performed from the mentioned cell line and after qualitative and quantitative evaluation of extracted total RNA, cDNA was synthesized. Then CXCL12 expression was measured using REAL TIME PCR.Results: CXCL12 low expression in lung adenocarcinoma A549 CELL LINE shows that it can be considered as a potential marker for predictive and therapeutic purposes.Conclusion: CXCLL12 has a low expression in A549 CELL LINE.

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APA: Copy

MAHBOBI, R., FALLAH MEHRABADI, J., & ALI ASGARI, E.. (2016). ASSESSMENT OF CXCL12 GENE EXPRESSION IN HUMAN LUNG ADENOCARCINOMA CELL LINE A549 BY REAL TIME PCR METHOD. JOURNAL OF ANIMAL PHYSIOLOGY AND DEVELOPMENT (QUARTERLY JOURNAL OF BIOLOGICAL SCIENCES), 9(3 (34)), 39-45. SID. https://sid.ir/paper/191698/en

Vancouver: Copy

MAHBOBI R., FALLAH MEHRABADI J., ALI ASGARI E.. ASSESSMENT OF CXCL12 GENE EXPRESSION IN HUMAN LUNG ADENOCARCINOMA CELL LINE A549 BY REAL TIME PCR METHOD. JOURNAL OF ANIMAL PHYSIOLOGY AND DEVELOPMENT (QUARTERLY JOURNAL OF BIOLOGICAL SCIENCES)[Internet]. 2016;9(3 (34)):39-45. Available from: https://sid.ir/paper/191698/en

IEEE: Copy

R. MAHBOBI, J. FALLAH MEHRABADI, and E. ALI ASGARI, “ASSESSMENT OF CXCL12 GENE EXPRESSION IN HUMAN LUNG ADENOCARCINOMA CELL LINE A549 BY REAL TIME PCR METHOD,” JOURNAL OF ANIMAL PHYSIOLOGY AND DEVELOPMENT (QUARTERLY JOURNAL OF BIOLOGICAL SCIENCES), vol. 9, no. 3 (34), pp. 39–45, 2016, [Online]. Available: https://sid.ir/paper/191698/en

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