Investigating the effect of biosynthetic nano-silver on HDAC8 expression in lung cancer A549 cell line

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Ranjbar T.; Asghari Moghaddam N.; Mohammadgholi A.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Feyz Medical Sciences Journal Year:2019 | Volume:22 | Issue:5 Page(s): 442-449

Download Full-Text

Persian Verion

View:

476

Download:

Cites:

Information Journal Paper

Title

Investigating the effect of biosynthetic nano-silver on HDAC8 expression in lung cancer A549 cell line

Author(s)

Ranjbar T. | Asghari Moghaddam N. | Mohammadgholi A. | Issue Writer Certificate

Keywords

Silver nanoparticlesQ1

Eucalyptus extractQ1

Lung cancerQ2

Cell line A549Q1

HDAC8Q1

Abstract

Background: Currently nanoparticles have encouraged a broad range of investigations in cancer therapy and diagnosis. The gene HDAC8 is responsible for repression of transcription in cells and it has been shown that its expression increases in Lung cancer. The aim of this study was to determine the effect of nanoparticles synthesized by the Eucalyptus extract on the expression of the HDAC8 gene in Lung cancer A549 cell line. Materials and Methods: In this experimental study, green synthesis of Silver nanoparticles was performed using the extract of Eucalyptus camaldulensis. In order to evaluate the half maximal inhibitory concentration (IC50), cultured A549 cells were treated by different concentrations of synthesized nanoparticles and the MTT assay was performed. Finally, the HDAC8 expression change of cells treated with the half maximal inhibitory concentration was determined by real-time PCR. Results: Results from the MTT assay revealed that the obtained nanoparticles decreased the viability of cells in concentration-dependent manner. The IC50 value of Silver nanoparticles in 24 hours against A549 cells was 2 μ g/ml. Also, the HDAC8 expression showed a 9-fold decrease in the treated cells. Conclusion: According to the results, nano-silver particles produced by the Eucalyptus extract can cause apoptosis by decreasing the HDAC8 expression. Hence, they can be considered as an agent with promising aspects to treat Lung cancer.

Cites

No record.

References

Cite

APA: Copy

Ranjbar, T., Asghari Moghaddam, N., & Mohammadgholi, A.. (2019). Investigating the effect of biosynthetic nano-silver on HDAC8 expression in lung cancer A549 cell line. FEYZ, 22(5 ), 442-449. SID. https://sid.ir/paper/40546/en

Vancouver: Copy

Ranjbar T., Asghari Moghaddam N., Mohammadgholi A.. Investigating the effect of biosynthetic nano-silver on HDAC8 expression in lung cancer A549 cell line. FEYZ[Internet]. 2019;22(5 ):442-449. Available from: https://sid.ir/paper/40546/en

IEEE: Copy

T. Ranjbar, N. Asghari Moghaddam, and A. Mohammadgholi, “Investigating the effect of biosynthetic nano-silver on HDAC8 expression in lung cancer A549 cell line,” FEYZ, vol. 22, no. 5 , pp. 442–449, 2019, [Online]. Available: https://sid.ir/paper/40546/en

Related Journal Papers