The Effect of Hydroalcholic extract Sargassum ciliforium on HT29 colorectal cancer cell line and evaluation P53 and APC genes expression By using Real time PCR Technique

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ZAHEDI A.; NAJI T.; AHMADI R.

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Paper Information

Journal: Journal of Molecular and Cellular Researches Year:2019 | Volume:32 | Issue:3 Page(s): 394-408

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Title

The Effect of Hydroalcholic extract Sargassum ciliforium on HT29 colorectal cancer cell line and evaluation P53 and APC genes expression By using Real time PCR Technique

Author(s)

ZAHEDI A. | NAJI T. | AHMADI R. | Issue Writer Certificate

Keywords

Colon cancerQ1

HT-29 cell lineQ1

Sargassum ciliforium algaeQ1

Real time PCRQ1

P53 and APC genesQ1

Abstract

Colon cancer is the third most common cancer in the world, with nearly 1. 4 million new cases diagnosed in 2012. In addition, the incidence of this disease has increased in recent years. Due to the increased incidence of cancer, the use of new chemotherapy molecules is required. Many studies have shown that intestinal cancers can be treated through natural marine products that contain a large amount of active biological substances with new chemical structures and new drug activities. The aim of this study was the effect of hydroalcoholic extract Sargassum ciliforium on HT-29 colorectal cell line and evaluation P53 and APC genes expression by using Real time PCR Technique. In this study, we determined Sargassum ciliforium extract with the MTT assay at 5 different concentrations 10-1-0. 1-0. 01-0. 001mg/ml and control group on HT-29 colorectal cancer cell line human embryonic kidney HEK were examined. Then optical density each well at wavelength 570nm it was found. Data analysis with spss software and One way Anova statistical test checked out. Also evaluation P53 and APC genes expression by using Real time PCR Technique. Our result showed that extract Sargassum ciliforium the effect reducation on viability in human Colon cancer cell line HT-29 and also prevent the growth of tumor cells. Results showed the extract Sargassum ciliforium had toxic effects in Colon cancer cell line HT-29 and this algae extract could be used to develope anti-cancer drugs and it was shown that induced cell death with Sargassum ciliforium algae extract through is the activation of APC protein.

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APA: Copy

ZAHEDI, A., NAJI, T., & AHMADI, R.. (2019). The Effect of Hydroalcholic extract Sargassum ciliforium on HT29 colorectal cancer cell line and evaluation P53 and APC genes expression By using Real time PCR Technique. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 32(3 ), 394-408. SID. https://sid.ir/paper/374371/en

Vancouver: Copy

ZAHEDI A., NAJI T., AHMADI R.. The Effect of Hydroalcholic extract Sargassum ciliforium on HT29 colorectal cancer cell line and evaluation P53 and APC genes expression By using Real time PCR Technique. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2019;32(3 ):394-408. Available from: https://sid.ir/paper/374371/en

IEEE: Copy

A. ZAHEDI, T. NAJI, and R. AHMADI, “The Effect of Hydroalcholic extract Sargassum ciliforium on HT29 colorectal cancer cell line and evaluation P53 and APC genes expression By using Real time PCR Technique,” JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 32, no. 3 , pp. 394–408, 2019, [Online]. Available: https://sid.ir/paper/374371/en

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