EFFECT OF SUPPLEMENTATION OF DIET WITH FERULA ASSAFOETIDAHYDOALKOHALIC EXTRACT ON IMMUNE RELATED (IL-1β AND TNF-ALPHA)

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SAFARI R.; VAHEDI AMIRI F.; SHABANY A.; HOSEINIFAR S.H.; KOLANGI MIANDAREH H.

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Journal: JOURNAL OF ANIMAL PHYSIOLOGY AND DEVELOPMENT (QUARTERLY JOURNAL OF BIOLOGICAL SCIENCES) Year:2018 | Volume:11 | Issue:3 (42) Page(s): 55-66

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Title

EFFECT OF SUPPLEMENTATION OF DIET WITH FERULA ASSAFOETIDAHYDOALKOHALIC EXTRACT ON IMMUNE RELATED (IL-1β AND TNF-ALPHA)

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Keywords

FERULA ASSAFOETIDAQ1

ZEBRAFISHQ1

GENE EXPRESSIONQ1

IL-1βAND TNF-α.Q1

Abstract

Inroduction & Objective: Due to the increased bacterial resistance to common antibiotics, there istendency towards using herbal extracts in order to increase the non-specific immune system. The aim ofpresent study was to investigate the effect of hydroalkohalic extract of FERULA ASSAFOETIDA on expression ofgenes related to immune (IL-1β and TNF-alpha) in ZEBRAFISH. Material and Methods: For this purpose ZEBRAFISH with average weight of 0. 3± 0. 1 gr, were fed dietssupplemented with 3 different levels of Ferula extract (0. 5, 1 and 2%) and one control group for 8 weeks. Atthe end of feeding trial, RNA extracted from intestine, cDNA syntesised and Real-time PCR were done usingIL-1β and TNF-alpha primers. Results: Results showed the increase in expression of both genes in fish fed 2% Ferula, which wassignificant with other group (0. 5% and 1%) in TNF-alpha (P<0. 05). IL-1β GENE EXPRESSION didn’ t showsignificant difference (P>0. 05) among fish fed ferula extracts, though the highest GENE EXPRESSION was seen in2% Ferula. Conclusion: According to results, it seems that using FERULA ASSAFOETIDA hydroalkohalic extract couldimprove ZEBRAFISH immune with increase of relative GENE EXPRESSION of inflammatory cytokine.

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APA: Copy

SAFARI, R., VAHEDI AMIRI, F., SHABANY, A., HOSEINIFAR, S.H., & KOLANGI MIANDAREH, H.. (2018). EFFECT OF SUPPLEMENTATION OF DIET WITH FERULA ASSAFOETIDAHYDOALKOHALIC EXTRACT ON IMMUNE RELATED (IL-1β AND TNF-ALPHA). JOURNAL OF ANIMAL PHYSIOLOGY AND DEVELOPMENT (QUARTERLY JOURNAL OF BIOLOGICAL SCIENCES), 11(3 (42) ), 55-66. SID. https://sid.ir/paper/191733/en

Vancouver: Copy

SAFARI R., VAHEDI AMIRI F., SHABANY A., HOSEINIFAR S.H., KOLANGI MIANDAREH H.. EFFECT OF SUPPLEMENTATION OF DIET WITH FERULA ASSAFOETIDAHYDOALKOHALIC EXTRACT ON IMMUNE RELATED (IL-1β AND TNF-ALPHA). JOURNAL OF ANIMAL PHYSIOLOGY AND DEVELOPMENT (QUARTERLY JOURNAL OF BIOLOGICAL SCIENCES)[Internet]. 2018;11(3 (42) ):55-66. Available from: https://sid.ir/paper/191733/en

IEEE: Copy

R. SAFARI, F. VAHEDI AMIRI, A. SHABANY, S.H. HOSEINIFAR, and H. KOLANGI MIANDAREH, “EFFECT OF SUPPLEMENTATION OF DIET WITH FERULA ASSAFOETIDAHYDOALKOHALIC EXTRACT ON IMMUNE RELATED (IL-1β AND TNF-ALPHA),” JOURNAL OF ANIMAL PHYSIOLOGY AND DEVELOPMENT (QUARTERLY JOURNAL OF BIOLOGICAL SCIENCES), vol. 11, no. 3 (42) , pp. 55–66, 2018, [Online]. Available: https://sid.ir/paper/191733/en

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