LUTETIUM LABELED-SIRNA DELIVERY INTO COLON CANCER CELLS USING LIPOSOMES: A COMPARATIVE STUDY

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FATHI M.; TAGHIKHANI M.; GHANNADI MARAGHEH M.; YAVARI K.

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Journal: NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL Year:2012 | Volume:2 | Issue:6 Page(s): 17-25

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Title

LUTETIUM LABELED-SIRNA DELIVERY INTO COLON CANCER CELLS USING LIPOSOMES: A COMPARATIVE STUDY

Author(s)

FATHI M. | TAGHIKHANI M. | GHANNADI MARAGHEH M. | YAVARI K. | Issue Writer Certificate

Keywords

TRANSFECTIONQ2

COLON CANCERQ1

IGF-1RQ2

SIRNAQ2

LUTETIUMQ2

CYTOTOXICQ2

Abstract

Aim and Background: Gene silencing effect of SIRNA significantly depends upon an appropriate method to introduce it into the cells. In the present study, the effect of liposomes on 177Lu-siRNA delivery into COLON CANCER cells has been studied quantitatively.Material and Methods: SIRNA was conjugated with p-SCN-Bn-DTPA and labeled with LUTETIUM.After being purified with Vivaspin and Sephadex G25 columns, 177Lu-siRNA complexes were evaluated for purity and integrity using ITLC and PAGE, respectively. Labeled SIRNAs were transfected into COLON CANCER cells using Lipofectamine and siPORT Neo FX. Then, percentage of the TRANSFECTION was quantitatively calculated with a gamma counter. For the evaluation of CYTOTOXIC effects of the labeled SIRNA, microscopic photographs and cell counting were used.Results: ITLC showed that the purity of 177Lu-siRNA complex products were in excess of 95%. The percentage of the transfected 177Lu-siRNA complexes with Lipofectamine was significantly higher compared to that of siPORT Neo FX (P<0.01). Cell counting and microscopic photographs indicated that 177lu-siRNA complex targeting IGF-1R had significantly CYTOTOXIC effect on the understudied cells compared to the control groups (P<0.001).Conclusion: The present study shows that efficiency of the lipofectamine in TRANSFECTION of the 177Lu-siRNA into COLON CANCER cells is more than siPORT Neo FX, and 177Lu-siRNA targeting IGF-1R has CYTOTOXIC effect on COLON CANCER cells.

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APA: Copy

FATHI, M., TAGHIKHANI, M., GHANNADI MARAGHEH, M., & YAVARI, K.. (2012). LUTETIUM LABELED-SIRNA DELIVERY INTO COLON CANCER CELLS USING LIPOSOMES: A COMPARATIVE STUDY. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, 2(6), 17-25. SID. https://sid.ir/paper/204667/en

Vancouver: Copy

FATHI M., TAGHIKHANI M., GHANNADI MARAGHEH M., YAVARI K.. LUTETIUM LABELED-SIRNA DELIVERY INTO COLON CANCER CELLS USING LIPOSOMES: A COMPARATIVE STUDY. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL[Internet]. 2012;2(6):17-25. Available from: https://sid.ir/paper/204667/en

IEEE: Copy

M. FATHI, M. TAGHIKHANI, M. GHANNADI MARAGHEH, and K. YAVARI, “LUTETIUM LABELED-SIRNA DELIVERY INTO COLON CANCER CELLS USING LIPOSOMES: A COMPARATIVE STUDY,” NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, vol. 2, no. 6, pp. 17–25, 2012, [Online]. Available: https://sid.ir/paper/204667/en

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