GENOTYPING OF PSEUDOMONAS AERUGINOSA STRAINS ISOLATED FROM TWO WARDS OF TALEGHANI HOSPITAL BY RAPD-PCR IN AHVAZ CITY OF IRAN

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NANVAZADEH FATEMEH; KHOSRAVI AZAR DOKHT; ZOLFAGHARI MOHAMMAD REZA; PARHIZGARI NAJMEH

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Journal: Jentashapir Journal of Cellular and Molecular Biology Year:2013 | Volume:- | Issue:SUPPLEMENT Page(s): 49-58

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Title

GENOTYPING OF PSEUDOMONAS AERUGINOSA STRAINS ISOLATED FROM TWO WARDS OF TALEGHANI HOSPITAL BY RAPD-PCR IN AHVAZ CITY OF IRAN

Author(s)

NANVAZADEH FATEMEH | KHOSRAVI AZAR DOKHT | ZOLFAGHARI MOHAMMAD REZA | PARHIZGARI NAJMEH | Issue Writer Certificate

Keywords

P. AERUGINOSAQ3

RAPD-PCRQ3

MOLECULAR GENOTYPINGQ3

Abstract

Background: Pseudomonas aeruginosa is a major health hazard particularly in immunodeficient patients, patients in ICU and burn units. It may be originated from different sources, and comprises a high colonization and transmission capacity. The present study aimed to investigate the genotypic variation of Pseudomonas aeroginosastrains isolated from burn patients using Random Amplified Polymorphic DNA (RAPD) method.Methods: Within 70 clinical samples collected from burn patients in Taleghani Burn Hospital, 44 samples were positive forP. AERUGINOSA by application of conventional tests. The RAPD-PCR method was applied according to standard method using a short single primer. The technique created repetitive electrophoresis patterns, which was used for genotypic differentiation.Results: Genetic fingerprinting of RAPD-PCR, created 13 different genotypic patterns with base pair length ranging from 180 to 2700. Each isolate roughly showed between three to six genotypic patterns. The patterns comprised nine genotypic diversity, designated as I to IX with genotype I as the most common, identified in 20% bacterial isolates. The least prevalent genotypes were V and VIII, identified in only 5% isolates.Conclusion: Based on the results, RAPD-PCR technique was found as an useful tool to investigate the genetic variation for P.aeruginosastrains. This technique is a rapid and low cost genotypic method with high discriminatory power. The results could assist the screening of original infection caused by P.aeruginosastraines with subsequent control of colonization and transmission.

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APA: Copy

NANVAZADEH, FATEMEH, KHOSRAVI, AZAR DOKHT, ZOLFAGHARI, MOHAMMAD REZA, & PARHIZGARI, NAJMEH. (2013). GENOTYPING OF PSEUDOMONAS AERUGINOSA STRAINS ISOLATED FROM TWO WARDS OF TALEGHANI HOSPITAL BY RAPD-PCR IN AHVAZ CITY OF IRAN. JENTASHAPIR JOURNAL OF CELLULAR AND MOLECULAR BIOLOGY (JENTASHAPIR JOURNAL OF HEALTH RESEARCH), -(SUPPLEMENT), 49-58. SID. https://sid.ir/paper/205743/en

Vancouver: Copy

NANVAZADEH FATEMEH, KHOSRAVI AZAR DOKHT, ZOLFAGHARI MOHAMMAD REZA, PARHIZGARI NAJMEH. GENOTYPING OF PSEUDOMONAS AERUGINOSA STRAINS ISOLATED FROM TWO WARDS OF TALEGHANI HOSPITAL BY RAPD-PCR IN AHVAZ CITY OF IRAN. JENTASHAPIR JOURNAL OF CELLULAR AND MOLECULAR BIOLOGY (JENTASHAPIR JOURNAL OF HEALTH RESEARCH)[Internet]. 2013;-(SUPPLEMENT):49-58. Available from: https://sid.ir/paper/205743/en

IEEE: Copy

FATEMEH NANVAZADEH, AZAR DOKHT KHOSRAVI, MOHAMMAD REZA ZOLFAGHARI, and NAJMEH PARHIZGARI, “GENOTYPING OF PSEUDOMONAS AERUGINOSA STRAINS ISOLATED FROM TWO WARDS OF TALEGHANI HOSPITAL BY RAPD-PCR IN AHVAZ CITY OF IRAN,” JENTASHAPIR JOURNAL OF CELLULAR AND MOLECULAR BIOLOGY (JENTASHAPIR JOURNAL OF HEALTH RESEARCH), vol. -, no. SUPPLEMENT, pp. 49–58, 2013, [Online]. Available: https://sid.ir/paper/205743/en

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