Product optimization of an extracellular alkalophilic organic solvent-resistant protease enzyme secreted by Bacillus sp.

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Mohamadi Shohreh; MEHRABI MARYAM

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF ANIMAL ENVIRONMENT Year:2020 | Volume:11 | Issue:4 Page(s): 397-408

Download Full-Text

Persian Verion

View:

521

Download:

Cites:

Information Journal Paper

Title

Product optimization of an extracellular alkalophilic organic solvent-resistant protease enzyme secreted by Bacillus sp.

Author(s)

Mohamadi Shohreh | MEHRABI MARYAM | Issue Writer Certificate

Keywords

ProteaseQ1

BacillusQ2

Organic solventQ1

Purification Q1

Abstract

The aim of this study was optimization of the bacterial growth and production of extracellular organic solvent-resistant Protease enzyme secreted by Bacillus sp. and the characterization of the enzyme. In this study, a species of Bacillus was isolated from a hot spring and grown in a medium enriched with cyclohexane (30%) and toluene (10%). Protease-producing bacteria were isolated using colonies grown on Skim Milk agar (SMA) plates. The Protease enzyme was purified in a two-step method including ammonium sulfate precipitation and DEAE-Sepharose anion exchange chromatography. Finally, one of the colonies was identified as the best strain with Protease activity. To optimize bacterial culture medium, various factors including maximum incubation time, temperature, pH, carbon and nitrogen sources were tested. The highest bacterial growth and Protease production were observed after 72 hours of incubation at 37 oC and pH 7 when the medium was supplemented with the 5% sucrose as a carbon source and yeast extract as a nitrogen source. This Protease showed the highest activity at 50 ° C and pH 10. It was inhibited by ethylene diamine tetraacetic acid (EDTA), but was not affected by serine Protease inhibitors, suggesting that the enzyme is a metalloprotease. Enzyme activity was increased in the presence of 10% (v / v) of toluene, methanol, ethanol and diethyl ether. Due to these properties, the enzyme can be used as a strong biocatalyst in the industrial and biotechnological applications.

Cites

No record.

References

No record.

Cite

APA: Copy

Mohamadi, Shohreh, & MEHRABI, MARYAM. (2020). Product optimization of an extracellular alkalophilic organic solvent-resistant protease enzyme secreted by Bacillus sp.. JOURNAL OF ANIMAL ENVIRONMENT, 11(4 ), 397-408. SID. https://sid.ir/paper/206598/en

Vancouver: Copy

Mohamadi Shohreh, MEHRABI MARYAM. Product optimization of an extracellular alkalophilic organic solvent-resistant protease enzyme secreted by Bacillus sp.. JOURNAL OF ANIMAL ENVIRONMENT[Internet]. 2020;11(4 ):397-408. Available from: https://sid.ir/paper/206598/en

IEEE: Copy

Shohreh Mohamadi, and MARYAM MEHRABI, “Product optimization of an extracellular alkalophilic organic solvent-resistant protease enzyme secreted by Bacillus sp.,” JOURNAL OF ANIMAL ENVIRONMENT, vol. 11, no. 4 , pp. 397–408, 2020, [Online]. Available: https://sid.ir/paper/206598/en

Related Journal Papers