ISOLATION, IDENTIFICATION AND CHARACTERIZATION OF ORGANIC SOLVENT TOLERANT PROTEASE FROM BACILLUS SP. DAF-01

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BADOEI DALFARD ARASTOO; AMIRI BAHRAMI MOSTAFA; RIAHI MADVAR ALI; KARAMI ZAHRA; EBRAHIMI MOHAMMAD ALI

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Journal: Journal of Microbial Biology Year:2012 | Volume:1 | Issue:2 Page(s): 37-47

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Title

ISOLATION, IDENTIFICATION AND CHARACTERIZATION OF ORGANIC SOLVENT TOLERANT PROTEASE FROM BACILLUS SP. DAF-01

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Keywords

PROTEASEQ2

ORGANIC SOLVENTSQ2

THERMOPILEQ2

SCREENINGQ2

Abstract

Introduction: Organic solvent-tolerant bacteria are relatively novel extermophilic microorganisms, which can produce organic tolerant PROTEASE with capacity of being used in industrial biotechnology for producing high-value compounds. Therefore, finding of these bacteria has drawn much researchers attention nowadays.Materials and Methods: In this project, samples were collected from a hot spring, located in Jiroft. Samples were incubated in medium supplemented with cyclohexane and toluene for 3 days. SCREENING of PROTEASE producing bacteria was performed on the specific media, SKM (Skim milk agar), based on clear area diameter. The best bacterium was identified based on 16s rDNA gene. PROTEASE activity was considered in different temperatures, pH and ORGANIC SOLVENTS.Results: Sequence alignment and phylogenetic tree results showed that this bacteria was closely related to Bacillus niacini, with 97% homology. Enzymatic studies showed that, this enzyme was active at a wide range of temperatures, 20-90 oC and it,s optimal activity was in 60 oC. In addition, maximum PROTEASE activity was obtained in the 8-9 range of pH, and optimal stability was also at pH 9.0. PROTEASE activity in the presence of methanol, toluene, isopropanol, cyclohexane and DMF showed that, remaining activity was at least 80% compared to the control (without organic solvent)Discussion and Conclusion: Thermopilic capacity, being active in alkaline PROTEASE and high PROTEASE stability in the presence of ORGANIC SOLVENTS all herald a remarkable application for using in different industries.

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APA: Copy

BADOEI DALFARD, ARASTOO, AMIRI BAHRAMI, MOSTAFA, RIAHI MADVAR, ALI, KARAMI, ZAHRA, & EBRAHIMI, MOHAMMAD ALI. (2012). ISOLATION, IDENTIFICATION AND CHARACTERIZATION OF ORGANIC SOLVENT TOLERANT PROTEASE FROM BACILLUS SP. DAF-01. BIOLOGICAL JOURNAL OF MICROORGANISM, 1(2), 37-47. SID. https://sid.ir/paper/237286/en

Vancouver: Copy

BADOEI DALFARD ARASTOO, AMIRI BAHRAMI MOSTAFA, RIAHI MADVAR ALI, KARAMI ZAHRA, EBRAHIMI MOHAMMAD ALI. ISOLATION, IDENTIFICATION AND CHARACTERIZATION OF ORGANIC SOLVENT TOLERANT PROTEASE FROM BACILLUS SP. DAF-01. BIOLOGICAL JOURNAL OF MICROORGANISM[Internet]. 2012;1(2):37-47. Available from: https://sid.ir/paper/237286/en

IEEE: Copy

ARASTOO BADOEI DALFARD, MOSTAFA AMIRI BAHRAMI, ALI RIAHI MADVAR, ZAHRA KARAMI, and MOHAMMAD ALI EBRAHIMI, “ISOLATION, IDENTIFICATION AND CHARACTERIZATION OF ORGANIC SOLVENT TOLERANT PROTEASE FROM BACILLUS SP. DAF-01,” BIOLOGICAL JOURNAL OF MICROORGANISM, vol. 1, no. 2, pp. 37–47, 2012, [Online]. Available: https://sid.ir/paper/237286/en

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