STUDY OF KIWIFRUIT ACTINIDIN USING SDS-PAGE, ISOELECTRIC FOCUSING AND TWO-DIMENSIONAL GEL ELECTROPHORESIS

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CHALABI M.; BAHRAMI Y.; MOSTAFAIE A.

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Journal: IRANIAN JOURNAL OF BIOLOGY Year:2004 | Volume:17 | Issue:3 Page(s): 219-226

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Title

STUDY OF KIWIFRUIT ACTINIDIN USING SDS-PAGE, ISOELECTRIC FOCUSING AND TWO-DIMENSIONAL GEL ELECTROPHORESIS

Author(s)

CHALABI M. | BAHRAMI Y. | MOSTAFAIE A. | Issue Writer Certificate

Keywords

ACTINIDINQ4

KIWIFIUITQ2

ELECTROPHORESISQ4

ISOELECTRIC FOCUSINGQ4

Abstract

ACTINIDIN is a thiol protease rich in KIWIFIUIT. This enzyme shows high degree of homology with other plant thiol proteases like papain. In this project ACTINIDIN was purified from Hayward cultivar of kiwi and characterized using SDS-PAGE, ISOELECTRIC FOCUSING (IEF) and Two-dimensional gel ELECTROPHORESIS (2DE).The enzyme was purified by salt fractionation and ion exchange chromatography and assayed upon casein hydrolysis method. SDS-PAGE was preformed in reducing and non-reducing conditions. IEF preformed in rehydrated gel using ampholine with pH range of 3.5-5 and 2DE carried out in slab gels in the two dimensions.Molecular mass of the purified enzymes were estimated 21, 26-27 or 29 KDa in different situations of SDS-PAGE. These results showed that treatment of enzyme with heat and reducing agent is necessary for complete unfolding and saturation with SDS. In IEF at least 6 isoenzymes with pI in range of 3.8-4.03 and one with pI of 3.5 were distinguished. Difference of these results with other similar studies can be resulting from differences of KIWIFIUIT cultivars and/or experimental methods. 2DE pattern of the purified enzyme showed considerable molecular mass differences between the isoenzymes. In conclusion, the information addressed in this project can help to better characterize ACTINIDIN isoenzymes and find their applications.

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APA: Copy

CHALABI, M., BAHRAMI, Y., & MOSTAFAIE, A.. (2004). STUDY OF KIWIFRUIT ACTINIDIN USING SDS-PAGE, ISOELECTRIC FOCUSING AND TWO-DIMENSIONAL GEL ELECTROPHORESIS. IRANIAN JOURNAL OF BIOLOGY, 17(3), 219-226. SID. https://sid.ir/paper/21017/en

Vancouver: Copy

CHALABI M., BAHRAMI Y., MOSTAFAIE A.. STUDY OF KIWIFRUIT ACTINIDIN USING SDS-PAGE, ISOELECTRIC FOCUSING AND TWO-DIMENSIONAL GEL ELECTROPHORESIS. IRANIAN JOURNAL OF BIOLOGY[Internet]. 2004;17(3):219-226. Available from: https://sid.ir/paper/21017/en

IEEE: Copy

M. CHALABI, Y. BAHRAMI, and A. MOSTAFAIE, “STUDY OF KIWIFRUIT ACTINIDIN USING SDS-PAGE, ISOELECTRIC FOCUSING AND TWO-DIMENSIONAL GEL ELECTROPHORESIS,” IRANIAN JOURNAL OF BIOLOGY, vol. 17, no. 3, pp. 219–226, 2004, [Online]. Available: https://sid.ir/paper/21017/en

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