ISOLATION AND PRIMARY CULTURE OF RAT HEPATOCYTES USING KIWIFRUIT ACTINIDIN

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SHIRVANI FARSANI Z.; MANSOURI K.; BIDMESHKIPOUR A.; MOSTAFAEI ALI

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Journal: Avicenna Journal of Clinical Medicine Year:2007 | Volume:14 | Issue:2 (SN 44) Page(s): 16-22

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Information Journal Paper

Title

ISOLATION AND PRIMARY CULTURE OF RAT HEPATOCYTES USING KIWIFRUIT ACTINIDIN

Author(s)

SHIRVANI FARSANI Z. | MANSOURI K. | BIDMESHKIPOUR A. | MOSTAFAEI ALI | Issue Writer Certificate

Keywords

ACTINIDINQ3

COLLAGENASEQ3

HEPATOCYTEQ3

Abstract

Introduction & Objective: Isolation of cells from different tissues rely on proteolytic enzymes mainly COLLAGENASEs that selectively digest collagen fibers of extra-cellular matrix. It is important to find new and proper COLLAGENASEs from plant sources. In the present research ACTINIDIN, a cysteine protease abundant in Kiwifruit, was used to isolate and culture of rat HEPATOCYTEs.Materials & Methods: Different concentrations of ACTINIDIN was used to isolate rat HEPATOCYTEs by one or two-step perfusion method. The viability of the separated cells was examined by the trypan blue test. The isolated rat HEPATOCYTEs were cultured on collagen coated plates in William's E medium. The morphology of HEPATOCYTEs was examined microscopically after staining with the Papanicolaou method.Results: ACTINIDIN in the concentration of 0.4 mg/ml in two-step perfusion method properly isolated HEPATOCYTEs from rat liver. The viability of isolated HEPATOCYTEs that successfully cultured in collagen coated plates was 90-95 percent.Conclusion: These results showed that ACTINIDIN is a proper protease for isolation of HEPATOCYTEs. In addition, purification of this enzyme is simpler than the COLLAGENASEs.

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References

KIWIFRUIT ACTINIDIN: ITS PURIFICATION AND EXAMINATION OF AMOUNT IN DIFFERENT VARIETIES

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APA: Copy

SHIRVANI FARSANI, Z., MANSOURI, K., BIDMESHKIPOUR, A., & MOSTAFAEI, ALI. (2007). ISOLATION AND PRIMARY CULTURE OF RAT HEPATOCYTES USING KIWIFRUIT ACTINIDIN. AVICENNA JOURNAL OF CLINICAL MEDICINE (SCIENTIFIC JOURNAL OF HAMADAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES), 14(2 (SN 44)), 16-22. SID. https://sid.ir/paper/17248/en

Vancouver: Copy

SHIRVANI FARSANI Z., MANSOURI K., BIDMESHKIPOUR A., MOSTAFAEI ALI. ISOLATION AND PRIMARY CULTURE OF RAT HEPATOCYTES USING KIWIFRUIT ACTINIDIN. AVICENNA JOURNAL OF CLINICAL MEDICINE (SCIENTIFIC JOURNAL OF HAMADAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES)[Internet]. 2007;14(2 (SN 44)):16-22. Available from: https://sid.ir/paper/17248/en

IEEE: Copy

Z. SHIRVANI FARSANI, K. MANSOURI, A. BIDMESHKIPOUR, and ALI MOSTAFAEI, “ISOLATION AND PRIMARY CULTURE OF RAT HEPATOCYTES USING KIWIFRUIT ACTINIDIN,” AVICENNA JOURNAL OF CLINICAL MEDICINE (SCIENTIFIC JOURNAL OF HAMADAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES), vol. 14, no. 2 (SN 44), pp. 16–22, 2007, [Online]. Available: https://sid.ir/paper/17248/en

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