PURIFICATION AND RECOVERY OF THERMOSTABLE ALKALINE PROTEASE IN AQUEOUS TWO-PHASE SYSTEMS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

MASHAYEKHI MAZAR F.; OMIDINIA E.; SHAHBAZ MOHAMADI H.; EBRAHEMI RAD M.; HOSSEINKHANI S.; GREGORIAN A.

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: IRANIAN JOURNAL OF BIOLOGY Year:2010 | Volume:23 | Issue:3 Page(s): 367-375

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

1,084

Download:

Cites:

Information Journal Paper

Title

PURIFICATION AND RECOVERY OF THERMOSTABLE ALKALINE PROTEASE IN AQUEOUS TWO-PHASE SYSTEMS

Author(s)

Keywords

AQUEOUS TWO-PHASE SYSTEMS (ATPS)Q3

PURIFICATIONQ2

THERMOSTABLE ALKALINE PROTEASEQ3

POLYETHYLENE GLYCOLQ2

SODIUM CITRATEQ3

Abstract

Thermostable alkaline proteases are a group of the most important industrial enzymes that have many applications due to function and withstand under harsh conditions such as alkaline pH ranges, high temperature and presence of chemical denaturants. In this case, isolation and screening of new enzymes with effective properties and also optimization of PURIFICATION methods is the first step in researching and expanding of these biocatalyst products. In this research, PURIFICATION and recovery of THERMOSTABLE ALKALINE PROTEASE from Bacillus subtilis strain in aqueous two-phase system (ATPS) containing POLYETHYLENE GLYCOL and SODIUM CITRATE was studied. The best conditions for enzyme PURIFICATION was obtained in system composed of 22% (w/w) PEG-l0000, 18% (w/w) SODIUM CITRATE and 12% (w/w) NaCI at pH 8.0. The PURIFICATION parameters such as partition coefficient of enzyme, yield, recovery, PURIFICATION factor and specific activity values were achieved 37.86, 97.77%, 177.5%, 27 and 3325.79 U/mg protein, respectively. The molecular weight of enzyme was estimated to be 41kDa by SDS-PAGE.The kinetic properties of Km and Vmax were also calculated as 2.21 mM and 0.26 D/ml, respectively.

Cites

No record.

References

No record.

Cite

APA: Copy

MASHAYEKHI MAZAR, F., OMIDINIA, E., SHAHBAZ MOHAMADI, H., EBRAHEMI RAD, M., HOSSEINKHANI, S., & GREGORIAN, A.. (2010). PURIFICATION AND RECOVERY OF THERMOSTABLE ALKALINE PROTEASE IN AQUEOUS TWO-PHASE SYSTEMS. IRANIAN JOURNAL OF BIOLOGY, 23(3), 367-375. SID. https://sid.ir/paper/21114/en

Vancouver: Copy

MASHAYEKHI MAZAR F., OMIDINIA E., SHAHBAZ MOHAMADI H., EBRAHEMI RAD M., HOSSEINKHANI S., GREGORIAN A.. PURIFICATION AND RECOVERY OF THERMOSTABLE ALKALINE PROTEASE IN AQUEOUS TWO-PHASE SYSTEMS. IRANIAN JOURNAL OF BIOLOGY[Internet]. 2010;23(3):367-375. Available from: https://sid.ir/paper/21114/en

IEEE: Copy

F. MASHAYEKHI MAZAR, E. OMIDINIA, H. SHAHBAZ MOHAMADI, M. EBRAHEMI RAD, S. HOSSEINKHANI, and A. GREGORIAN, “PURIFICATION AND RECOVERY OF THERMOSTABLE ALKALINE PROTEASE IN AQUEOUS TWO-PHASE SYSTEMS,” IRANIAN JOURNAL OF BIOLOGY, vol. 23, no. 3, pp. 367–375, 2010, [Online]. Available: https://sid.ir/paper/21114/en

Related Journal Papers