PURIFICATION AND RECOVERY OF THERMOSTABLE ALKALINE PROTEASE IN AQUEOUS TWO-PHASE SYSTEMS

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MASHAYEKHI MAZAR F.; OMIDINIA E.; SHAHBAZ MOHAMADI H.; EBRAHEMI RAD M.; HOSSEINKHANI S.; GREGORIAN A.

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Journal: IRANIAN JOURNAL OF BIOLOGY Year:2010 | Volume:23 | Issue:3 Page(s): 367-375

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Title

PURIFICATION AND RECOVERY OF THERMOSTABLE ALKALINE PROTEASE IN AQUEOUS TWO-PHASE SYSTEMS

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Keywords

AQUEOUS TWO-PHASE SYSTEMS (ATPS)Q3

PURIFICATIONQ2

THERMOSTABLE ALKALINE PROTEASEQ3

POLYETHYLENE GLYCOLQ2

SODIUM CITRATEQ3

Abstract

Thermostable alkaline proteases are a group of the most important industrial enzymes that have many applications due to function and withstand under harsh conditions such as alkaline pH ranges, high temperature and presence of chemical denaturants. In this case, isolation and screening of new enzymes with effective properties and also optimization of PURIFICATION methods is the first step in researching and expanding of these biocatalyst products. In this research, PURIFICATION and recovery of THERMOSTABLE ALKALINE PROTEASE from Bacillus subtilis strain in aqueous two-phase system (ATPS) containing POLYETHYLENE GLYCOL and SODIUM CITRATE was studied. The best conditions for enzyme PURIFICATION was obtained in system composed of 22% (w/w) PEG-l0000, 18% (w/w) SODIUM CITRATE and 12% (w/w) NaCI at pH 8.0. The PURIFICATION parameters such as partition coefficient of enzyme, yield, recovery, PURIFICATION factor and specific activity values were achieved 37.86, 97.77%, 177.5%, 27 and 3325.79 U/mg protein, respectively. The molecular weight of enzyme was estimated to be 41kDa by SDS-PAGE.The kinetic properties of Km and Vmax were also calculated as 2.21 mM and 0.26 D/ml, respectively.

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APA: Copy

MASHAYEKHI MAZAR, F., OMIDINIA, E., SHAHBAZ MOHAMADI, H., EBRAHEMI RAD, M., HOSSEINKHANI, S., & GREGORIAN, A.. (2010). PURIFICATION AND RECOVERY OF THERMOSTABLE ALKALINE PROTEASE IN AQUEOUS TWO-PHASE SYSTEMS. IRANIAN JOURNAL OF BIOLOGY, 23(3), 367-375. SID. https://sid.ir/paper/21114/en

Vancouver: Copy

MASHAYEKHI MAZAR F., OMIDINIA E., SHAHBAZ MOHAMADI H., EBRAHEMI RAD M., HOSSEINKHANI S., GREGORIAN A.. PURIFICATION AND RECOVERY OF THERMOSTABLE ALKALINE PROTEASE IN AQUEOUS TWO-PHASE SYSTEMS. IRANIAN JOURNAL OF BIOLOGY[Internet]. 2010;23(3):367-375. Available from: https://sid.ir/paper/21114/en

IEEE: Copy

F. MASHAYEKHI MAZAR, E. OMIDINIA, H. SHAHBAZ MOHAMADI, M. EBRAHEMI RAD, S. HOSSEINKHANI, and A. GREGORIAN, “PURIFICATION AND RECOVERY OF THERMOSTABLE ALKALINE PROTEASE IN AQUEOUS TWO-PHASE SYSTEMS,” IRANIAN JOURNAL OF BIOLOGY, vol. 23, no. 3, pp. 367–375, 2010, [Online]. Available: https://sid.ir/paper/21114/en

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