Study of Quantitative and Qualitative of B1, B2, G1 and G2 Aflatoxins in Two Species of Aspergillus With HPLC Method

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ASGHARNEZHAD F.; MAHDIAN S.; AMIRI BESHELI B.; SEYED JAFAR NAZARI S.S.

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Journal: Journal of Applied Research in Plant Protection Year:2016 | Volume:5 | Issue:2 Page(s): 149-160

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Title

Study of Quantitative and Qualitative of B1, B2, G1 and G2 Aflatoxins in Two Species of Aspergillus With HPLC Method

Author(s)

ASGHARNEZHAD F. | MAHDIAN S. | AMIRI BESHELI B. | SEYED JAFAR NAZARI S.S. | Issue Writer Certificate

Keywords

AspergillusQ2

AflatoxinQ2

Food safetyQ2

High Performance Liquid ChromatographyQ1

Abstract

Aflatoxins are the fungal toxins produced by Aspergillus spp. These fungal metabolites in food and oil seed have a big lifetime risk for acute and chronic disease in human and animals. In this survey for production levels of B1, B2, G1 and G2 Aflatoxins in two species of Aspergillus was namely A. Alaus and A. Foctides has been investigated. These fungi were grown in Potato Dextrose Broth (PDB) medium and Aflatoxins were determined using HPLC method. These species were cultured in 50 ml flask at 26º c, with 80% humidity and dark place for 32 days. 25 ml of broth cultures were filtered and then extracted with 10 ml chloroform and evaporated the organic solvent and bring the organic solvent in 3ml methanol. After filtration, 20 μ l of final solvent was injected to HPLC instrument. The result has shown that A. foetidus produced the maximum amount of B1, B2 and G1 with 8. 71, 2. 3 & 3. 1 mg after 24 days post-inoculation and the maximum amount of G2 with 0. 02 after 20 days post-inoculation per 50 ml of PDB. A. flavus produced the maximum amount of B1, B2 and G2 with 487. 92, 18. 46 & 8. 57 mg after 20 days post-inoculation and the maximum amount of G1 with 126. 49 mg after 24 days post-inoculation per 50 ml of PDB. Overall the B1 is maximum afltoxin in A. flavus and G2 is the minimum Aflatoxin in A. foetidus.

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APA: Copy

ASGHARNEZHAD, F., MAHDIAN, S., AMIRI BESHELI, B., & SEYED JAFAR NAZARI, S.S.. (2016). Study of Quantitative and Qualitative of B1, B2, G1 and G2 Aflatoxins in Two Species of Aspergillus With HPLC Method. JOURNAL OF APPLIED RESEARCHES IN PLANT PROTECTION, 5(2 ), 149-160. SID. https://sid.ir/paper/237622/en

Vancouver: Copy

ASGHARNEZHAD F., MAHDIAN S., AMIRI BESHELI B., SEYED JAFAR NAZARI S.S.. Study of Quantitative and Qualitative of B1, B2, G1 and G2 Aflatoxins in Two Species of Aspergillus With HPLC Method. JOURNAL OF APPLIED RESEARCHES IN PLANT PROTECTION[Internet]. 2016;5(2 ):149-160. Available from: https://sid.ir/paper/237622/en

IEEE: Copy

F. ASGHARNEZHAD, S. MAHDIAN, B. AMIRI BESHELI, and S.S. SEYED JAFAR NAZARI, “Study of Quantitative and Qualitative of B1, B2, G1 and G2 Aflatoxins in Two Species of Aspergillus With HPLC Method,” JOURNAL OF APPLIED RESEARCHES IN PLANT PROTECTION, vol. 5, no. 2 , pp. 149–160, 2016, [Online]. Available: https://sid.ir/paper/237622/en

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