ISOLATION AND IDENTIFICATION OF GIARDIA LAMBLIA CYSTS FROM SURFACE WATERS OF RASHT CITY

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

SAEEDI E.; JONEIDI JAFARI N.; SALEH ZADEH A.; KAZEMI R.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download Full-Text

Persian Verion

View:

1,134

Download:

Cites:

Information Journal Paper

Title

ISOLATION AND IDENTIFICATION OF GIARDIA LAMBLIA CYSTS FROM SURFACE WATERS OF RASHT CITY

Author(s)

SAEEDI E. | JONEIDI JAFARI N. | SALEH ZADEH A. | KAZEMI R. | Issue Writer Certificate

Keywords

GIARDIA LAMBLIAQ1

CYSTSQ1

PCRQ1

RASHTQ1

Abstract

Introduction: GIARDIA LAMBLIA is a protozoan parasite with global spread in the human population.Identification, diagnosis and determination of the incidence of the infectious agent can be valuable to control the possible sources of spread of this infectious agent in human communities. Therefore, the aim of this study was isolation and diagnosis of Giardia CYSTS from surface waters of RASHT based on microscopic and molecular methods.Methods: This Cross-sectional study was performed on 45 samples of surface water collected from rivers and wetlands in the vicinity of RASHT. The samples were concentrated using nitrocellulose membrane filters. Giardia CYSTS were sought in concentrated deposits using Lugol staining. GIARDIA LAMBLIA genome amplification was done by specific primers on DNA extracted from sediment samples using phenol-chloroform method.Results: From 45 surface water samples, 15 (33.33%) were positive based on concentration and microscopic observation and 18 (40%) were positive in PCR. PCR had 100% sensitivity and 90% specificity in comparison with the standard staining method.Conclusion: In detection of GIARDIA LAMBLIA CYSTS from concentrated water samples, PCR is more sensitive and specific than microscopy. Given the common use of water resources for agriculture, use of sanitary methods of pathogen control including preventing human sewage from entering into any water source is recommended.

Cites

No record.

References

No record.

Cite

APA: Copy

SAEEDI, E., JONEIDI JAFARI, N., SALEH ZADEH, A., & KAZEMI, R.. (2017). ISOLATION AND IDENTIFICATION OF GIARDIA LAMBLIA CYSTS FROM SURFACE WATERS OF RASHT CITY. JOURNAL OF NURSE AND PHYSICIAN WITHIN WAR, 5(16 ), 19-24. SID. https://sid.ir/paper/246143/en

Vancouver: Copy

SAEEDI E., JONEIDI JAFARI N., SALEH ZADEH A., KAZEMI R.. ISOLATION AND IDENTIFICATION OF GIARDIA LAMBLIA CYSTS FROM SURFACE WATERS OF RASHT CITY. JOURNAL OF NURSE AND PHYSICIAN WITHIN WAR[Internet]. 2017;5(16 ):19-24. Available from: https://sid.ir/paper/246143/en

IEEE: Copy

E. SAEEDI, N. JONEIDI JAFARI, A. SALEH ZADEH, and R. KAZEMI, “ISOLATION AND IDENTIFICATION OF GIARDIA LAMBLIA CYSTS FROM SURFACE WATERS OF RASHT CITY,” JOURNAL OF NURSE AND PHYSICIAN WITHIN WAR, vol. 5, no. 16 , pp. 19–24, 2017, [Online]. Available: https://sid.ir/paper/246143/en

Related Journal Papers