PROTEOMIC ANALYSIS OF TRANSGENICARABIDOPSIS PLANTS OVER EXPRESSINGGR-RBP2 IN COMPARISON WITH WILD TYPE

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

AGHDASI M.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Molecular and Cellular Researches Year:2013 | Volume:26 | Issue:2 Page(s): 154-163

Download Full-Text

Persian Verion

View:

747

Download:

Cites:

Information Journal Paper

Title

PROTEOMIC ANALYSIS OF TRANSGENICARABIDOPSIS PLANTS OVER EXPRESSINGGR-RBP2 IN COMPARISON WITH WILD TYPE

Author(s)

AGHDASI M. | Issue Writer Certificate

Keywords

GR-RBP2Q2

PROTEOMIC ANALYSISQ2

MITOCHONDRIAQ2

GENE TRANSFORMATIONQ2

Abstract

Glycine-Rich RNA Binding Protein2 (GR-RBP2) is one of the eight GR-RBP family members in Arabidopsis thaliana which is located in MITOCHONDRIA after translation. Here we employed a proteomic approach to compare transgenic Arabidopsis plants over expressing GR-RBP2 with Wild Type. In this study, GR-RBP2 gene was isolated from Arabidopsis and cloned into pBIN19 vector. Recombinant vector was transferred to Arabidopsis (Col-0) via Agrobacterium tumefaciensby Floral Dipping method. Transformation with full length cDNA of GR-RBP2 yielded 21 lines. Over expressing plants are indistinguishable from WT seedlings. The whole lines are unchanged with regard to flowering time and are fully fertile. Gene expression levels were determined in different over expressing lines compare with WT seedlings. The mRNA level in over expressing lines was 2 times higher than WT. PROTEOMIC ANALYSIS of over expressing lines were carried out on two different gel systems: 2D IEF/ SDA PAGE and 2D Blue-native/SDS PAGE. All gels were silver stained. 2D gel electrophoresis revealed that GRRBP2 was induced in over expressing lines. Results showed that respiratory chain looks very much in the same investigated lines and WT. Complex V was partially degraded (F1 complex), but this also is the same in all samples. Approximately 110 spots were identified by mass spectrometry. Six protein spots were increased in over expressing lines compared to WT. A look at some well known proteins showed that there is no major change indicating induction or repression of metabolic pathways.

Cites

No record.

References

Cite

APA: Copy

AGHDASI, M.. (2013). PROTEOMIC ANALYSIS OF TRANSGENICARABIDOPSIS PLANTS OVER EXPRESSINGGR-RBP2 IN COMPARISON WITH WILD TYPE. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 26(2), 154-163. SID. https://sid.ir/paper/248588/en

Vancouver: Copy

AGHDASI M.. PROTEOMIC ANALYSIS OF TRANSGENICARABIDOPSIS PLANTS OVER EXPRESSINGGR-RBP2 IN COMPARISON WITH WILD TYPE. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2013;26(2):154-163. Available from: https://sid.ir/paper/248588/en

IEEE: Copy

M. AGHDASI, “PROTEOMIC ANALYSIS OF TRANSGENICARABIDOPSIS PLANTS OVER EXPRESSINGGR-RBP2 IN COMPARISON WITH WILD TYPE,” JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 26, no. 2, pp. 154–163, 2013, [Online]. Available: https://sid.ir/paper/248588/en

Related Journal Papers