AN EVALUATION ON ELASTASE ENZYME ACTIVITY IN GINGIVAL CREVICULAR FLUID IN PERIODONTITIS

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GHOUJEGH D.; AMOUEIAN B.; ZABIHI P.

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Journal: Journal of Dental Medicine Year:2003 | Volume:16 | Issue:3 (36) Page(s): 72-78

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Title

AN EVALUATION ON ELASTASE ENZYME ACTIVITY IN GINGIVAL CREVICULAR FLUID IN PERIODONTITIS

Author(s)

GHOUJEGH D. | AMOUEIAN B. | ZABIHI P. | Issue Writer Certificate

Keywords

GINGIVAL CREVICULAR FLUIDQ4

ELASTASEQ4

PERIODONTAL DISEASEQ2

Abstract

Statement of Problem: Changes in protein levels, host calls enzymes and inflammatory mediators in GINGIVAL CREVICULAR FLUID (GCF) are considered as diagnostic indicators of Periodontitis.Purpose: he aim of the present study was to measure the ELASTASE enzyme activity in GINGIVAL CREVICULAR FLUID among patients with periodontitis. Material and Methods: In this study, 52 periodontitis patients (experimental group) and 51 healthy subjects without any gingival inflammatio (control group) were participated. Subjects of the periodontitis group showed pockets of 4-5 mm depth without gingival enlargement and recession or pockets of 1-2 mm depth with gingival recession. For enzyme activity measurement, 100µl of gingival fluid of each sample was mixed with 100µl of enzyme substrate on the tube. the mixture was incubated at 34°c for 1h with a buffer solution of 1ml volume and absorbance was read at 410nm with spectrophotometer. The enzyme activity differences between two groups were analyzed by student t test.Results: The ELASTASE enzyme activity in GINGIVAL CREVICULAR FLUID in subjects with periodontium destruction and control subjects was 153±11.3 and 52.7±10.4 enzyme unit in ml per minute, respectively. The difference between groups was statistically significant (P<0.05).Conclusion: Based on the findings of this study, the measurement of elastae enzyme activity could be a useful indication of tissue changes that may ultimately manifest clinically as periodontitis.

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APA: Copy

GHOUJEGH, D., AMOUEIAN, B., & ZABIHI, P.. (2003). AN EVALUATION ON ELASTASE ENZYME ACTIVITY IN GINGIVAL CREVICULAR FLUID IN PERIODONTITIS. JOURNAL OF DENTAL MEDICINE, 16(3 (36)), 72-78. SID. https://sid.ir/paper/25217/en

Vancouver: Copy

GHOUJEGH D., AMOUEIAN B., ZABIHI P.. AN EVALUATION ON ELASTASE ENZYME ACTIVITY IN GINGIVAL CREVICULAR FLUID IN PERIODONTITIS. JOURNAL OF DENTAL MEDICINE[Internet]. 2003;16(3 (36)):72-78. Available from: https://sid.ir/paper/25217/en

IEEE: Copy

D. GHOUJEGH, B. AMOUEIAN, and P. ZABIHI, “AN EVALUATION ON ELASTASE ENZYME ACTIVITY IN GINGIVAL CREVICULAR FLUID IN PERIODONTITIS,” JOURNAL OF DENTAL MEDICINE, vol. 16, no. 3 (36), pp. 72–78, 2003, [Online]. Available: https://sid.ir/paper/25217/en

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