ANTIPROLIFERATION EFFECT OF HEAT KILLED INDIGENOUS PROBIOTIC BACTERIA ON AGS CELL LINE

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RASULI ELNAZ; TAFVIZI FARZANEH; Tajabady Ebrahimi maryam

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Journal: JOURNAL OF APPLIED MICROBIOLOGY IN FOOD INDUSTRY Year:2017 | Volume:3 | Issue:1 Page(s): 42-52

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Information Journal Paper

Title

ANTIPROLIFERATION EFFECT OF HEAT KILLED INDIGENOUS PROBIOTIC BACTERIA ON AGS CELL LINE

Author(s)

RASULI ELNAZ | TAFVIZI FARZANEH | Tajabady Ebrahimi maryam | Issue Writer Certificate

Keywords

CYTOTOXICQ1

GASTRIC CANCER HEAT-KILLED PROBIOTIC BACTERIAQ1

Abstract

Aopoptosis induction is one of the best method cancer therapy and funding the anti-cancer component, especially inducing opoptosis is of the significant important. This study aimed to assess CYTOTOXIC effects of heat-killed probiotic bacteria and induced apoptosis on gastric cancer AGS cells compared to normal cells (HEK-293). AGS cancer cell line AGS was cultured in RPMI medium, and cell line HEK-239 was selected as the control. Both cell lines received 0.01, 0.1, 1, 10, 100, and 1000 mg/ml of heat-killed lactobacillus paracasei for periods of 24, 48, and 72 hours. Viability of cells was measured using MTT assay. Apoptosis assay was performed in IC50 of cell culture by Annexin V and propidium iodide staining methods in 48 hours. According to MTT assay, heat-killed bacteria reduced viability and proliferation of gastric cancer cell line AGS in pattern of time, dose and strain dependent. The highest CYTOTOXIC effect was observed in 1000 mg/ml for 72 hours. Annexin V staining and flow cytometry confirmed induced apoptosis in AGS cell line in pattern of time, dose and strain dependent. Heat-killed probiotic bacteria showed CYTOTOXIC and induced apoptosis effects on AGS gastric cancer cell line, with slight CYTOTOXIC effect on HEK-239 compared to AGS. Further studies are still required to confirm these bacteria as a biological anti-cancer product in treatment and prevention.

Cites

THE EFFECT OF PROBIOTICS ON SIGNALING PATHWAYS OF GENESINVOLVED IN DEPRESSION: REVIEW ARTICLE

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APA: Copy

RASULI, ELNAZ, TAFVIZI, FARZANEH, & Tajabady Ebrahimi, maryam. (2017). ANTIPROLIFERATION EFFECT OF HEAT KILLED INDIGENOUS PROBIOTIC BACTERIA ON AGS CELL LINE. JOURNAL OF APPLIED MICROBIOLOGY IN FOOD INDUSTRY, 3(1), 42-52. SID. https://sid.ir/paper/254850/en

Vancouver: Copy

RASULI ELNAZ, TAFVIZI FARZANEH, Tajabady Ebrahimi maryam. ANTIPROLIFERATION EFFECT OF HEAT KILLED INDIGENOUS PROBIOTIC BACTERIA ON AGS CELL LINE. JOURNAL OF APPLIED MICROBIOLOGY IN FOOD INDUSTRY[Internet]. 2017;3(1):42-52. Available from: https://sid.ir/paper/254850/en

IEEE: Copy

ELNAZ RASULI, FARZANEH TAFVIZI, and maryam Tajabady Ebrahimi, “ANTIPROLIFERATION EFFECT OF HEAT KILLED INDIGENOUS PROBIOTIC BACTERIA ON AGS CELL LINE,” JOURNAL OF APPLIED MICROBIOLOGY IN FOOD INDUSTRY, vol. 3, no. 1, pp. 42–52, 2017, [Online]. Available: https://sid.ir/paper/254850/en

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