IMMUNOHISTOCHEMICAL STUDY OF DISTRIBUTION OF GAD AND GABA IN THE ENTORHINAL CORTEX AFTER SPREADING DEPRESSION IN JUVENILE RATS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

LOTFINIA AHMAD ALI; ALIZADEH LEILA; LOTFINIA MAHMOUD; SAJADIAN AZADEH; GHAEMI AMIR

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Neuroscience Journal of Shefaye Khatam Year:2015 | Volume:3 | Issue:2 Page(s): 27-34

Download Full-Text

Persian Verion

View:

803

Download:

Cites:

Information Journal Paper

Title

IMMUNOHISTOCHEMICAL STUDY OF DISTRIBUTION OF GAD AND GABA IN THE ENTORHINAL CORTEX AFTER SPREADING DEPRESSION IN JUVENILE RATS

Author(s)

Keywords

CORTICAL SPREADING DEPRESSIONQ3

ENTORHINAL CORTEXQ3

GAMMA-AMINOBUTYRIC ACIDQ3

Abstract

Introduction: Spreading depression (SD), discovered by Leao in 1944, is a pathophysiological depolarization wave that propagates slowly in the brain (3 mm/min) and causes dramatic ionic and hemodynamic changes. SD appears to act through several mechanisms and receptors, which have not completely understood. Here, we studied the effect of inhibitory system in animal model of SD using immunohistochemistry technique.Materials and Methods: After implanting recording electrodes and cannula over the brain, repetitive SD was induced by KCl injection (2 M) in juvenile rats for four consecutive weeks. Then all rats’ brains removed. The mean number of dark neurons in the ENTORHINAL CORTEX (EC) were determined using toluidine blue staining. To identify the prevalence and distribution of GABAA subunit receptors as well as glutamic acid decarboxylase (GAD), the GABA biosynthetic enzyme, immunohistochemistry technique was performed.Results: The mean number of SD induced was statistically increased during four weeks of experiments. The mean number of dark neurons in EC was significantly increased in SD group compared to sham group. Furthermore, expression of GAD65 in EC significantly increased in SD group compared to sham group. However, both GABA-Aα and GABA-Aβ subunit receptors didn’t significantly change in that region after SD.Conclusion: These data suggest that SD is able to damage the neuronal cells in neural tissues in juvenile rats and parallelly lead to enhancement of GAD65 in the central nervous system.

Cites

No record.

References

No record.

Cite

APA: Copy

LOTFINIA, AHMAD ALI, ALIZADEH, LEILA, LOTFINIA, MAHMOUD, SAJADIAN, AZADEH, & GHAEMI, AMIR. (2015). IMMUNOHISTOCHEMICAL STUDY OF DISTRIBUTION OF GAD AND GABA IN THE ENTORHINAL CORTEX AFTER SPREADING DEPRESSION IN JUVENILE RATS. NEUROSCIENCE JOURNAL OF SHEFAYE KHATAM, 3(2), 27-34. SID. https://sid.ir/paper/258027/en

Vancouver: Copy

LOTFINIA AHMAD ALI, ALIZADEH LEILA, LOTFINIA MAHMOUD, SAJADIAN AZADEH, GHAEMI AMIR. IMMUNOHISTOCHEMICAL STUDY OF DISTRIBUTION OF GAD AND GABA IN THE ENTORHINAL CORTEX AFTER SPREADING DEPRESSION IN JUVENILE RATS. NEUROSCIENCE JOURNAL OF SHEFAYE KHATAM[Internet]. 2015;3(2):27-34. Available from: https://sid.ir/paper/258027/en

IEEE: Copy

AHMAD ALI LOTFINIA, LEILA ALIZADEH, MAHMOUD LOTFINIA, AZADEH SAJADIAN, and AMIR GHAEMI, “IMMUNOHISTOCHEMICAL STUDY OF DISTRIBUTION OF GAD AND GABA IN THE ENTORHINAL CORTEX AFTER SPREADING DEPRESSION IN JUVENILE RATS,” NEUROSCIENCE JOURNAL OF SHEFAYE KHATAM, vol. 3, no. 2, pp. 27–34, 2015, [Online]. Available: https://sid.ir/paper/258027/en

Related Journal Papers