Comparing Supportive Properties of Poly Lactic-Co-Glycolic Acid (PLGA), PLGA/Collagen and Human Amniotic Membrane for Human Urothelial and Smooth Muscle Cells Engineering

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Sharifiaghdas Farzaneh; NAJI MOHAMMAD; SARHANGNEJAD REZA; Rajabi Zeleti Sareh; MIRZADEH HAMID; ZANDI MOJGAN; Saeed Mahdi

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Journal: UROLOGY JOURNAL Year:2014 | Volume:11 | Issue:3 Page(s): 1620-1628

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Title

Comparing Supportive Properties of Poly Lactic-Co-Glycolic Acid (PLGA), PLGA/Collagen and Human Amniotic Membrane for Human Urothelial and Smooth Muscle Cells Engineering

Author(s)

Keywords

biocompatible materials

cell proliferation

membranes

artificial

polyglycolic acid

urinary bladder

regeneration

tissue engineering

amnion

muscle

smooth

cell culture techniques

Abstract

Purpose: To compare human urothelial and smooth muscle cells attachment and proliferation using three different matrices; poly lactic-co-glycolic acid (PLGA), PLGA/collagen and human amniotic membrane (hAM). Materials and Methods: Human urothelial and smooth muscle cells were cultured and examined for expression of urothelium (pancytokeratin and uroplakin III) and smooth muscle cells [desmin and alpha smooth muscle actin (α-SMA)] markers. Cells were cultured on three scaffolds; PLGA, PLGA/collagen and hAM. Thereafter, they were analyzed for cell growth on days 1, 3, 7, 14 and 21 after seeding by 3-(4, 5-dimethylthiazole-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Scaffolds were fixed and processed for hematoxylin and eosin (H&E) staining and immunohistochemistry against their cell specific markers after 7 and 14 days of culture. Results: MTT assay results revealed that collagen has improved cell attachment features of PLGA and led to significant increase of MTT signal in PLGA/collagen compared to PLGA (P <. 001) and hAM (P <. 001). hAM was a weaker matrix for both cell types as demonstrated in MTT assay and scanning electron microscope (SEM) images. SEM micrographs showed normal phenotype and distribution on PLGA and PLGA/collagen. In the same line, cells formed a well-developed layer either on PLGA or PLGA/collagen, which maintained expression of their corresponding markers. Conclusion: Our findings demonstrated significant improvement of cell attachment and growth achieved by collagen coating (PLGA/collagen) compared to PLGA and hAM. hAM despite of its natural entity was a weaker matrix for bladder engineering purposes.

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APA: Copy

Sharifiaghdas, Farzaneh, NAJI, MOHAMMAD, SARHANGNEJAD, REZA, Rajabi Zeleti, Sareh, MIRZADEH, HAMID, ZANDI, MOJGAN, & Saeed, Mahdi. (2014). Comparing Supportive Properties of Poly Lactic-Co-Glycolic Acid (PLGA), PLGA/Collagen and Human Amniotic Membrane for Human Urothelial and Smooth Muscle Cells Engineering. UROLOGY JOURNAL, 11(3), 1620-1628. SID. https://sid.ir/paper/271108/en

Vancouver: Copy

Sharifiaghdas Farzaneh, NAJI MOHAMMAD, SARHANGNEJAD REZA, Rajabi Zeleti Sareh, MIRZADEH HAMID, ZANDI MOJGAN, Saeed Mahdi. Comparing Supportive Properties of Poly Lactic-Co-Glycolic Acid (PLGA), PLGA/Collagen and Human Amniotic Membrane for Human Urothelial and Smooth Muscle Cells Engineering. UROLOGY JOURNAL[Internet]. 2014;11(3):1620-1628. Available from: https://sid.ir/paper/271108/en

IEEE: Copy

Farzaneh Sharifiaghdas, MOHAMMAD NAJI, REZA SARHANGNEJAD, Sareh Rajabi Zeleti, HAMID MIRZADEH, MOJGAN ZANDI, and Mahdi Saeed, “Comparing Supportive Properties of Poly Lactic-Co-Glycolic Acid (PLGA), PLGA/Collagen and Human Amniotic Membrane for Human Urothelial and Smooth Muscle Cells Engineering,” UROLOGY JOURNAL, vol. 11, no. 3, pp. 1620–1628, 2014, [Online]. Available: https://sid.ir/paper/271108/en

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