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Information Journal Paper

Title

EFFECT OF MERCURIC CHLORIDE ON KINETIC PROPERTIES OF HORSERADISH PEROXIDASE

Pages

  49-56

Abstract

 Mercury is one of the three major environmental metal poisons, and MERCURIC CHLORIDE is a highly reactive compound which can harm cells by a variety of mechanisms including direct interaction with sulphydryl groups of proteins and enzymes, therefore affecting the enzymatic activity. This study focused on the effect of Hg++ on HORSERADISH PEROXIDASE (donor: hydrogen peroxide oxidoreductase, EC 1.11.1.7) (HRP) (Isoenzyme C) activity. In the presence of 88 mM hydrogen peroxide Km for o-dianisidine oxidation was 0.05 millimolar and Vmax was 8.5 μM.s-1. Incubation of the enzyme with 1 to 100 millimolar MERCURIC CHLORIDE for 5-20- and 60 min resulted in progressive inhibition of the enzymatic activity. At low Hg++ concentrations the inhibition was reversible by excess substrate, while at high Hg++ concentration the inhibition was not reversible. Results also indicated that the type of inhibition depended on the duration of incubation of the enzyme with metal ion and on the Hg++ concentration. So we could conclude that the type of inhibition changed from noncompetitive to mix with increased incubation time and increased metal concentration.

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    Cite

    APA: Copy

    EYN ELAHI, N., ABBASI, S., & VAEZ ZADEH, F.. (2006). EFFECT OF MERCURIC CHLORIDE ON KINETIC PROPERTIES OF HORSERADISH PEROXIDASE. IRANIAN JOURNAL OF PUBLIC HEALTH, 35(2), 49-56. SID. https://sid.ir/paper/272240/en

    Vancouver: Copy

    EYN ELAHI N., ABBASI S., VAEZ ZADEH F.. EFFECT OF MERCURIC CHLORIDE ON KINETIC PROPERTIES OF HORSERADISH PEROXIDASE. IRANIAN JOURNAL OF PUBLIC HEALTH[Internet]. 2006;35(2):49-56. Available from: https://sid.ir/paper/272240/en

    IEEE: Copy

    N. EYN ELAHI, S. ABBASI, and F. VAEZ ZADEH, “EFFECT OF MERCURIC CHLORIDE ON KINETIC PROPERTIES OF HORSERADISH PEROXIDASE,” IRANIAN JOURNAL OF PUBLIC HEALTH, vol. 35, no. 2, pp. 49–56, 2006, [Online]. Available: https://sid.ir/paper/272240/en

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