ACID DIGESTION AND MICROPLATE READING METHOD FOR MILK IODINE DETERMINATION

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HEDAYATI S.M.; ORDOUKHANI A.; DANESHPOUR M.A.S.; AZIZI FEREYDOUN

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Journal: IRANIAN JOURNAL OF ENDOCRINOLOGY AND METABOLISM (IJEM) Year:2007 | Volume:8 | Issue:4 (SN 32) Page(s): 357-363

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Title

ACID DIGESTION AND MICROPLATE READING METHOD FOR MILK IODINE DETERMINATION

Author(s)

HEDAYATI S.M. | ORDOUKHANI A. | DANESHPOUR M.A.S. | AZIZI FEREYDOUN | Issue Writer Certificate

Keywords

ACID DIGESTIONQ3

MILK IODINEQ3

MICROPLATE READINGQ3

Abstract

Introduction: Main shortcomings in existing methods for iodine determination in milk samples are non safe alkaline solution, harsh thermal conditions, and their being time consuming. In this study, for determination of total iodine content in milk, a simple and rapid kinetic catalytic colorimetric, ACID DIGESTION and rapid MICROPLATE READING format method, was investigated.Materials and Methods: Sample digestion was done on 50mL milk in metavanadate/perchloric acid, at 230oC for 10 min. After digestion, iodine determination was based on famous Sandell – Kolthoff reaction. The reaction results were read in 96 wells microplate by ELISA reader.Results: Work range of the assay was between 2-40mg/dl. The within-run coefficient of variation percent ranged from 6. 7 to 9 3 and between-run coefficients of variation ranged from 8.6 to 12.3%.The results obtained (n=70) by the optimized method had good correlation with the results of alkaline incineration as the reference method (p<0.000, n=70; r2=0.907; y=0. 952x-0.084). Recovery tests for accuracy assessment were between 91.3 to 113%. This method enabled us to achieve 0.1 mg/dl sensitivity. Conclusion: This study showed that, fast ACID DIGESTION, mild thermal, fast results reading and low sample volume, were the main advantages of the ACID DIGESTION and MICROPLATE READING format investigated.

Cites

تاثیر سطوح ید بالاتر از مقادیر توصیه شده NRC بر عملکرد و غلظت هورمون های تیروئیدی گاوهای هولشتاین

References

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APA: Copy

HEDAYATI, S.M., ORDOUKHANI, A., DANESHPOUR, M.A.S., & AZIZI, FEREYDOUN. (2007). ACID DIGESTION AND MICROPLATE READING METHOD FOR MILK IODINE DETERMINATION. IRANIAN JOURNAL OF ENDOCRINOLOGY AND METABOLISM (IJEM), 8(4 (SN 32)), 357-363. SID. https://sid.ir/paper/27327/en

Vancouver: Copy

HEDAYATI S.M., ORDOUKHANI A., DANESHPOUR M.A.S., AZIZI FEREYDOUN. ACID DIGESTION AND MICROPLATE READING METHOD FOR MILK IODINE DETERMINATION. IRANIAN JOURNAL OF ENDOCRINOLOGY AND METABOLISM (IJEM)[Internet]. 2007;8(4 (SN 32)):357-363. Available from: https://sid.ir/paper/27327/en

IEEE: Copy

S.M. HEDAYATI, A. ORDOUKHANI, M.A.S. DANESHPOUR, and FEREYDOUN AZIZI, “ACID DIGESTION AND MICROPLATE READING METHOD FOR MILK IODINE DETERMINATION,” IRANIAN JOURNAL OF ENDOCRINOLOGY AND METABOLISM (IJEM), vol. 8, no. 4 (SN 32), pp. 357–363, 2007, [Online]. Available: https://sid.ir/paper/27327/en

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