Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

260
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

140
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

THE EFFECT OF SNAIL1 GENE SILENCING BY SIRNA IN METASTATIC BREAST CANCER CELL LINES

Pages

  659-670

Abstract

 Background: BREAST CANCER is the most common diagnosed cancer among women in the world. SNAIL1 plays a role in the development of the invasive phenotypes of cancer, neural cell differentiation, cell division and apoptosis in tumor cells. Traces of SNAIL1 in metastasis of BREAST CANCER to bone are observed. The aim of this study was to investigate the effect of specific SNAIL1 SIRNAs on the proliferation, migration, induction of apoptosis and cell cycle arrest of MDA-MB-468 cells.Methods: In 2015, this experimental study was performed on the MDA-MB-468 CELL LINEs in Immunology Research Center, Tabriz University of Medical Sciences. After the design and construction of SIRNA, transfection was per-formed with transfection reagent. The expression levels of mRNA and protein were measured by qRT-PCR and west-ern blot analysis, respectively. The survival of cells was determined by using MTT assay cells, apoptosis using Tunel assay, Cell migration using scratch assay, Cell cycle analysis by Propidium Iodide (PI) DNA staining method using flow cytometry on the MDA-MB-468.Results: Transfection with SIRNA significantly suppressed the expression of SNAIL1 gene in dose-dependent manner after 48 h (P<0.0001). Surprisingly, treatment with SNAIL1 SIRNA arrested cell cycle in S phases (P<0.0001). Moreover, SIRNA transfection had effects on breast adenocarcinoma cells and inhibited the migration (P<0.0001), proliferation (P<0.0001) and induced apoptosis (P<0.0016).Conclusion: The SNAIL1 can be considered as a potent adjuvant in BREAST CANCER therapy.

Cites

  • No record.

    • References

  • No record.

    • Cite

    APA: Copy

    ALETAHA, MANSOOR, MANSOORI, BEHZAD, MOHAMMADI, ALI, FAZELI, MEHDI, & BARADARAN, BEHZAD. (2017). THE EFFECT OF SNAIL1 GENE SILENCING BY SIRNA IN METASTATIC BREAST CANCER CELL LINES. IRANIAN JOURNAL OF PUBLIC HEALTH, 46(5), 659-670. SID. https://sid.ir/paper/274140/en

    Vancouver: Copy

    ALETAHA MANSOOR, MANSOORI BEHZAD, MOHAMMADI ALI, FAZELI MEHDI, BARADARAN BEHZAD. THE EFFECT OF SNAIL1 GENE SILENCING BY SIRNA IN METASTATIC BREAST CANCER CELL LINES. IRANIAN JOURNAL OF PUBLIC HEALTH[Internet]. 2017;46(5):659-670. Available from: https://sid.ir/paper/274140/en

    IEEE: Copy

    MANSOOR ALETAHA, BEHZAD MANSOORI, ALI MOHAMMADI, MEHDI FAZELI, and BEHZAD BARADARAN, “THE EFFECT OF SNAIL1 GENE SILENCING BY SIRNA IN METASTATIC BREAST CANCER CELL LINES,” IRANIAN JOURNAL OF PUBLIC HEALTH, vol. 46, no. 5, pp. 659–670, 2017, [Online]. Available: https://sid.ir/paper/274140/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top