SIMULTANEOUS DETERMINATION OF ANTHOCYANOSIDE AND BETA-CAROTENE BY THIRD-DERIVATIVE ULTRAVIOLET SPECTROPHOTOMETRY

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SOURI E.; JALALIZADEH H.; FARSAM H.; REZWANI H.; AMANLOU M.

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Title

SIMULTANEOUS DETERMINATION OF ANTHOCYANOSIDE AND BETA-CAROTENE BY THIRD-DERIVATIVE ULTRAVIOLET SPECTROPHOTOMETRY

Author(s)

Keywords

ANTHOCYANOSIDE

BETA-CAROTENE

THIRD DERIVATIVE SPECTROPHOTOMETRY

SIMULTANEOUS

Abstract

Derivative spectrophotometry offers a useful approach for the analysis of drugs in multi-component mixtures. In this study a third-derivative spectrophotometry method was used for SIMULTANEOUS determination of ANTHOCYANOSIDE and BETA-CAROTENE using the zero-crossing technique. The measurements were carried out at wavelengths of 625 and 540 nm for ANTHOCYANOSIDE and BETA-CAROTENE respectively.The method was found to be linear (r2>0.999) in the range of 125-750 µg/mL for ANTHOCYANOSIDE in the presence of 25 µg/mL BETA-CAROTENE at 625 nm. The same linear correlation was also obtained (r2>0.997) in the range of 6.25-37.50 µg/mL for BETA-CAROTENE in the presence of 500 µg/mL of ANTHOCYANOSIDE at 540 nm. The limit of determination was 125 and 6.25 µg/mL for ANTHOCYANOSIDE and BETA-CAROTENE respectively. The method was successfully applied for SIMULTANEOUS determination of ANTHOCYANOSIDE and BETA-CAROTENE in pharmaceutical preparations without any interferences from excipients.

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APA: Copy

SOURI, E., JALALIZADEH, H., FARSAM, H., REZWANI, H., & AMANLOU, M.. (2005). SIMULTANEOUS DETERMINATION OF ANTHOCYANOSIDE AND BETA-CAROTENE BY THIRD-DERIVATIVE ULTRAVIOLET SPECTROPHOTOMETRY. DARU JOURNAL OF PHARMACEUTICAL SCIENCE, 13(1), 11-16. SID. https://sid.ir/paper/275235/en

Vancouver: Copy

SOURI E., JALALIZADEH H., FARSAM H., REZWANI H., AMANLOU M.. SIMULTANEOUS DETERMINATION OF ANTHOCYANOSIDE AND BETA-CAROTENE BY THIRD-DERIVATIVE ULTRAVIOLET SPECTROPHOTOMETRY. DARU JOURNAL OF PHARMACEUTICAL SCIENCE[Internet]. 2005;13(1):11-16. Available from: https://sid.ir/paper/275235/en

IEEE: Copy

E. SOURI, H. JALALIZADEH, H. FARSAM, H. REZWANI, and M. AMANLOU, “SIMULTANEOUS DETERMINATION OF ANTHOCYANOSIDE AND BETA-CAROTENE BY THIRD-DERIVATIVE ULTRAVIOLET SPECTROPHOTOMETRY,” DARU JOURNAL OF PHARMACEUTICAL SCIENCE, vol. 13, no. 1, pp. 11–16, 2005, [Online]. Available: https://sid.ir/paper/275235/en

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