مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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1

Information Journal Paper

Title

SIMULTANEOUS DETERMINATION OF METOPROLOL, PROPRANOLOL AND PHENOL RED IN SAMPLES FROM RAT IN SITU INTESTINAL PERFUSION STUDIES

Pages

  102-108

Abstract

 Single-pass intestinal perfusion technique (SPIP) is the most used classic technique employed in the study of intestinal absorption of compounds in which a non-absorbable marker such as PHENOL RED is used to correct the water flux. A simple and rapid reversed-phase high performance liquid chromatographic method with UV detection at 227 nm was developed for simultaneous quantitation of PROPRANOLOL and METOPROLOL along with PHENOL RED for in-situ PERMEABILITY studies. The mobile phase was a mixture of 55% methanol, 45% of 0.05 M KH2PO4 aqueous solution (adjusted to pH 6) and 0.2 % (v/v) triethylamine. Analysis was run at a flow rate of 1 ml/min with a 9 min run time. The calibration curves were linear for all three compounds (r > 0.999) across the concentration range of 7.5-125 μg/ml with a limit of detection of 4.24, 2.18 and 8.57 ng/ml and limit of quantification of 14, 7.2 and 28.3 ng/ml for METOPROLOL, PROPRANOLOL and PHENOL RED respectively. The coefficient of variation for intra-assay and interassay precision was less than 8% and the accuracy was between 93.6-107%. Using the SPIP technique and the suggested HPLC method for sample analysis, the mean values of 0.49 e-4 (±0.19) cm/sec and 0.32 e-4 (± 0.09) cm/sec were obtained for PROPRANOLOL and METOPROLOL intestinal PERMEABILITY coefficients respectively.

Cites

References

Cite

APA: Copy

ZAKERI MILANI, P., VALIZADEH, H., AZARMI, Y.A., BARZEGAR JALALI, M., & TAJERZADEH, H.. (2006). SIMULTANEOUS DETERMINATION OF METOPROLOL, PROPRANOLOL AND PHENOL RED IN SAMPLES FROM RAT IN SITU INTESTINAL PERFUSION STUDIES. DARU JOURNAL OF PHARMACEUTICAL SCIENCE, 14(2), 102-108. SID. https://sid.ir/paper/275338/en

Vancouver: Copy

ZAKERI MILANI P., VALIZADEH H., AZARMI Y.A., BARZEGAR JALALI M., TAJERZADEH H.. SIMULTANEOUS DETERMINATION OF METOPROLOL, PROPRANOLOL AND PHENOL RED IN SAMPLES FROM RAT IN SITU INTESTINAL PERFUSION STUDIES. DARU JOURNAL OF PHARMACEUTICAL SCIENCE[Internet]. 2006;14(2):102-108. Available from: https://sid.ir/paper/275338/en

IEEE: Copy

P. ZAKERI MILANI, H. VALIZADEH, Y.A. AZARMI, M. BARZEGAR JALALI, and H. TAJERZADEH, “SIMULTANEOUS DETERMINATION OF METOPROLOL, PROPRANOLOL AND PHENOL RED IN SAMPLES FROM RAT IN SITU INTESTINAL PERFUSION STUDIES,” DARU JOURNAL OF PHARMACEUTICAL SCIENCE, vol. 14, no. 2, pp. 102–108, 2006, [Online]. Available: https://sid.ir/paper/275338/en

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