ASSESSMENT OF GM-CSF RECEPTORS BY REAL–TIME RT-PCR ON CELL LINES EXPRESSING HIGH AND LOW AFFINITY RECEPTORS AND THEIR RELATION TO CYTOTOXIC EFFECT OF CHIMERIC PROTEIN (STXA1-GM-CSF)

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HABIBI ROUDKENAR M.; BOUZARI S.; OLOUMI MANA; KUWAHARA Y.; MOHAMMADI ROUSHANDEH A.; BABA T.; FUKUMOTO M.

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Journal Paper

Paper Information

Journal: DARU Journal of Pharmaceutical Sciences Year:2007 | Volume:15 | Issue:3 Page(s): 151-155

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Title

ASSESSMENT OF GM-CSF RECEPTORS BY REAL–TIME RT-PCR ON CELL LINES EXPRESSING HIGH AND LOW AFFINITY RECEPTORS AND THEIR RELATION TO CYTOTOXIC EFFECT OF CHIMERIC PROTEIN (STXA1-GM-CSF)

Author(s)

Keywords

STXA1-GM-CSF

GM-CSF RECEPTOR

CYTOTOXICITY

REAL–TIME RT-PCR

Abstract

Immunotoxins, which are composed of both the cell targeting and the cell killing moieties are the new approach for targeted therapy of human disease .In all immunotoxins that GM-CSF has been used as cell targeting; only cell lines expressing high affinity receptor have been used for CYTOTOXICITY studies. In the present study, various cell lines expressing high and low affinity receptors were used for assessment of the cytotoxic effect of hybrid chimeric protein. The expression of GM-CSF RECEPTOR (GM-CSFR) was quantified by real-time RT- PCR. The cell lines K562 and THP1 expressing high affinity receptor and MC-7, PC-3 and DU145 expressing low affinity receptor were used for this study. The chimeric hybrid protein was found to be toxic for various cell lines used in this investigation and CYTOTOXICITY was more effective in cell lines bearing high affinity receptors. Overall, our results showed that the recombinant hybrid protein could have wide range of application on various cancer cell lines even cells bearing low affinity receptors for GM-CSF.

Cites

LIPOCALIN 2 ACTS AS A CYTOPROTECTIVE FACTOR AGAINST CISPLATIN TOXICITY, AN IN VITRO STUDY

References

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APA: Copy

HABIBI ROUDKENAR, M., BOUZARI, S., OLOUMI, MANA, KUWAHARA, Y., MOHAMMADI ROUSHANDEH, A., BABA, T., & FUKUMOTO, M.. (2007). ASSESSMENT OF GM-CSF RECEPTORS BY REAL–TIME RT-PCR ON CELL LINES EXPRESSING HIGH AND LOW AFFINITY RECEPTORS AND THEIR RELATION TO CYTOTOXIC EFFECT OF CHIMERIC PROTEIN (STXA1-GM-CSF). DARU JOURNAL OF PHARMACEUTICAL SCIENCE, 15(3), 151-155. SID. https://sid.ir/paper/275409/en

Vancouver: Copy

HABIBI ROUDKENAR M., BOUZARI S., OLOUMI MANA, KUWAHARA Y., MOHAMMADI ROUSHANDEH A., BABA T., FUKUMOTO M.. ASSESSMENT OF GM-CSF RECEPTORS BY REAL–TIME RT-PCR ON CELL LINES EXPRESSING HIGH AND LOW AFFINITY RECEPTORS AND THEIR RELATION TO CYTOTOXIC EFFECT OF CHIMERIC PROTEIN (STXA1-GM-CSF). DARU JOURNAL OF PHARMACEUTICAL SCIENCE[Internet]. 2007;15(3):151-155. Available from: https://sid.ir/paper/275409/en

IEEE: Copy

M. HABIBI ROUDKENAR, S. BOUZARI, MANA OLOUMI, Y. KUWAHARA, A. MOHAMMADI ROUSHANDEH, T. BABA, and M. FUKUMOTO, “ASSESSMENT OF GM-CSF RECEPTORS BY REAL–TIME RT-PCR ON CELL LINES EXPRESSING HIGH AND LOW AFFINITY RECEPTORS AND THEIR RELATION TO CYTOTOXIC EFFECT OF CHIMERIC PROTEIN (STXA1-GM-CSF),” DARU JOURNAL OF PHARMACEUTICAL SCIENCE, vol. 15, no. 3, pp. 151–155, 2007, [Online]. Available: https://sid.ir/paper/275409/en

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