EXPRESSION OF A CHIMERIC PROTEIN CONTAINING THE CATALYTIC DOMAIN OF SHIGA-LIKE TOXIN AND HUMAN GRANULOCYTE MACROPHAGE COLONY-STIMULATING FACTOR (HGM-CSF) IN ESCHERICHIA COLI AND ITS RECOGNITION BY RECIPROCAL ANTIBODIES

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HABIBI ROUDKENAR M.; BOUZARI S.; OLOUMI MANA; JAFARI A.A.F.; SHAHROKHI N.; SHOKRGOZAR M.A.

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Journal Paper

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Journal: Iranian Biomedical Journal Year:2005 | Volume:9 | Issue:4 Page(s): 143-148

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Title

EXPRESSION OF A CHIMERIC PROTEIN CONTAINING THE CATALYTIC DOMAIN OF SHIGA-LIKE TOXIN AND HUMAN GRANULOCYTE MACROPHAGE COLONY-STIMULATING FACTOR (HGM-CSF) IN ESCHERICHIA COLI AND ITS RECOGNITION BY RECIPROCAL ANTIBODIES

Author(s)

Keywords

EXPRESSION

CHIMERIC PROTEIN

SHIGA-TOXIN

HUMAN GRANULOCYTE MACROPHAGE COLONY STIMULATING FACTOR (HGM-CSF)

ESCHERICHIA COLI

Abstract

Fusion of two genes at DNA level produces a single protein, known as a CHIMERIC PROTEIN. Immunotoxins are CHIMERIC PROTEINs composed of specific cell targeting and cell killing moieties. Bacterial or plant toxins are commonly used as the killing moieties of the chimeric immunotoxins. In this investigation, the catalytic domain of Shiga-like toxin (A1) was fused to human granulocyte macrophage colony stimulating factor (hGM-CSF) gene and the fused gene was then expressed using an EXPRESSION vector containing arabinose promoter. The protein thus obtained could be recognized by two different ELISA system designed for detection of hGM-CSF and SHIGA-TOXIN and reconfirmed by Western-blot. The recognition of the CHIMERIC PROTEIN by specific antibodies could be indicative of the proper form of the protein, which justifies further steps to be taken to evaluate the potential effects of the CHIMERIC PROTEIN

Cites

ASSESSMENT OF GM-CSF RECEPTORS BY REAL–TIME RT-PCR ON CELL LINES EXPRESSING HIGH AND LOW AFFINITY RECEPTORS AND THEIR RELATION TO CYTOTOXIC EFFECT OF CHIMERIC PROTEIN (STXA1-GM-CSF)

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APA: Copy

HABIBI ROUDKENAR, M., BOUZARI, S., OLOUMI, MANA, JAFARI, A.A.F., SHAHROKHI, N., & SHOKRGOZAR, M.A.. (2005). EXPRESSION OF A CHIMERIC PROTEIN CONTAINING THE CATALYTIC DOMAIN OF SHIGA-LIKE TOXIN AND HUMAN GRANULOCYTE MACROPHAGE COLONY-STIMULATING FACTOR (HGM-CSF) IN ESCHERICHIA COLI AND ITS RECOGNITION BY RECIPROCAL ANTIBODIES. IRANIAN BIOMEDICAL JOURNAL, 9(4), 143-148. SID. https://sid.ir/paper/277102/en

Vancouver: Copy

HABIBI ROUDKENAR M., BOUZARI S., OLOUMI MANA, JAFARI A.A.F., SHAHROKHI N., SHOKRGOZAR M.A.. EXPRESSION OF A CHIMERIC PROTEIN CONTAINING THE CATALYTIC DOMAIN OF SHIGA-LIKE TOXIN AND HUMAN GRANULOCYTE MACROPHAGE COLONY-STIMULATING FACTOR (HGM-CSF) IN ESCHERICHIA COLI AND ITS RECOGNITION BY RECIPROCAL ANTIBODIES. IRANIAN BIOMEDICAL JOURNAL[Internet]. 2005;9(4):143-148. Available from: https://sid.ir/paper/277102/en

IEEE: Copy

M. HABIBI ROUDKENAR, S. BOUZARI, MANA OLOUMI, A.A.F. JAFARI, N. SHAHROKHI, and M.A. SHOKRGOZAR, “EXPRESSION OF A CHIMERIC PROTEIN CONTAINING THE CATALYTIC DOMAIN OF SHIGA-LIKE TOXIN AND HUMAN GRANULOCYTE MACROPHAGE COLONY-STIMULATING FACTOR (HGM-CSF) IN ESCHERICHIA COLI AND ITS RECOGNITION BY RECIPROCAL ANTIBODIES,” IRANIAN BIOMEDICAL JOURNAL, vol. 9, no. 4, pp. 143–148, 2005, [Online]. Available: https://sid.ir/paper/277102/en

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