A RAPID AND SENSITIVE MODIFIED HPLC METHOD FOR DETERMINATION OF DICLOFENAC IN HUMAN PLASMA AND ITS APPLICATION IN PHARMACOKINETIC STUDIES

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EMAMI J.; GHASEMI N.; TALARI R.

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Journal Paper

Paper Information

Journal: DARU Journal of Pharmaceutical Sciences Year:2007 | Volume:15 | Issue:3 Page(s): 132-138

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Title

A RAPID AND SENSITIVE MODIFIED HPLC METHOD FOR DETERMINATION OF DICLOFENAC IN HUMAN PLASMA AND ITS APPLICATION IN PHARMACOKINETIC STUDIES

Author(s)

EMAMI J. | GHASEMI N. | TALARI R. | Issue Writer Certificate

Keywords

CHROMATOGRAPHY

HPLC

BIOEQUIVALENCE

DICLOFENAC

Abstract

A sensitive, accurate and rapid reverse phase HPLC method was developed to quantitate plasma levels of DICLOFENAC sodium in human plasma. The drug, internal standard (naproxene) and orthophosphoric acid 1 M, were added to plasma samples and vortexed for 20 sec. A mixture of hexane /isopropyl alcohol (90:10) was then added and vortexed for 2 min. Samples were centrifuged and the supernatant layer was separated, evaporated to dryness under nitrogen gas stream, reconstituted in mobile phase and an aliquot of 50 ml was analyzed on a m-bondapack C18 (150×4.6mm) column, with 45% acetonitrile in deionised water and 0.5% orthophosphoric acid, (pH=3.5) at 276 nm. The standard curve covering 0.005 - 4 mg/ml concentration range, was linear, relative errors were within 0.13 to 16 % and the CV% ranged from 1.24 to 8.75. The limits of quantitation and detection of the method were 0.005 mg/mL and 0.002mg/mL, respectively. The method was suitable for bioavailability and pharmacokinetic studies of DICLOFENAC in humans and applied in a randomized, two-way cross over bioequivalance study of two different DICLOFENAC sodium preparations with twelve subjects and with a one-week washout period.

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APA: Copy

EMAMI, J., GHASEMI, N., & TALARI, R.. (2007). A RAPID AND SENSITIVE MODIFIED HPLC METHOD FOR DETERMINATION OF DICLOFENAC IN HUMAN PLASMA AND ITS APPLICATION IN PHARMACOKINETIC STUDIES. DARU JOURNAL OF PHARMACEUTICAL SCIENCE, 15(3), 132-138. SID. https://sid.ir/paper/275412/en

Vancouver: Copy

EMAMI J., GHASEMI N., TALARI R.. A RAPID AND SENSITIVE MODIFIED HPLC METHOD FOR DETERMINATION OF DICLOFENAC IN HUMAN PLASMA AND ITS APPLICATION IN PHARMACOKINETIC STUDIES. DARU JOURNAL OF PHARMACEUTICAL SCIENCE[Internet]. 2007;15(3):132-138. Available from: https://sid.ir/paper/275412/en

IEEE: Copy

J. EMAMI, N. GHASEMI, and R. TALARI, “A RAPID AND SENSITIVE MODIFIED HPLC METHOD FOR DETERMINATION OF DICLOFENAC IN HUMAN PLASMA AND ITS APPLICATION IN PHARMACOKINETIC STUDIES,” DARU JOURNAL OF PHARMACEUTICAL SCIENCE, vol. 15, no. 3, pp. 132–138, 2007, [Online]. Available: https://sid.ir/paper/275412/en

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