CYTOPLASMIC EXPRESSION OF RECOMBINANT INTERLEUKIN-2 AND INTERLEUKIN-4 PROTEINS RESULTS IN HYDROGEN PEROXIDE ACCUMULATION AND REDUCTION IN CATALASE ACTIVITY IN ESCHERICHIA COLI

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

HEJAZI M.S.; KARIMI F.; MAHDIZADEH AGHDAM E.; BARZEGARI A.; FARSH DOUSTI HAGH M.; PARVIZI M.; MAHMOUDI AZAR L.; HEJAZI M.A.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: DARU Journal of Pharmaceutical Sciences Year:2009 | Volume:17 | Issue:2 Page(s): 64-71

Download Full-Text

View:

370

Download:

173

Cites:

Information Journal Paper

Title

CYTOPLASMIC EXPRESSION OF RECOMBINANT INTERLEUKIN-2 AND INTERLEUKIN-4 PROTEINS RESULTS IN HYDROGEN PEROXIDE ACCUMULATION AND REDUCTION IN CATALASE ACTIVITY IN ESCHERICHIA COLI

Author(s)

Keywords

HYDROGEN PEROXIDE

CATALASE ACTIVITY

CYTOPLASMIC EXPRESSION

ESCHERICHIA COLI

RECOMBINANT PROTEIN

Abstract

Background and the purpose of the study: The Reactive oxygen species (ROS) is induced in the cells following various stresses but the effect of RECOMBINANT PROTEIN expression on ROS generation has not been studied yet. In this study, H2O2 concentration and CATALASE ACTIVITY variations and their correlation with cell growth following CYTOPLASMIC EXPRESSION of human interleukin-2 (hIL-2) and mouse interleukin-4 (mIL-4) in ESCHERICHIA COLI were investigated. Additionally, the effect of RECOMBINANT PROTEIN expression under different conditions was compared to the effect of foreign DNA introduction on these factors.Methods: Plasmids pEThIL-2 and pETmIL-4 were used for expression of human interleukin-2 (hIL-2) and mouse interleukin-4 (mIL-4) inside the cytoplasm of the cells. Having confirmed protein expression using SDS-PAGE analysis and ELISA assay, H2O2 concentration and CATALASE ACTIVITY were measured at various ODs.Results and major conclusion: Empty vector introduction increased significantly H2O2 concentration of the cells. However, H2O2 concentration in hIL-2 and mIL-4 expressing cells was significantly higher than its concentration in empty vector transformed cells. CATALASE ACTIVITY was reduced in foreign DNA introduced cells. It was more lowered following expression of RECOMBINANT PROTEINs. Results of this study revealed the relationship between foreign DNA introduction and protein expression with H2O2 elevation and CATALASE ACTIVITY reduction. There was also correlation between H2O2 elevation and reduction in CATALASE ACTIVITY with the cell growth depression.

Cites

No record.

References

No record.

Cite

APA: Copy

HEJAZI, M.S., KARIMI, F., MAHDIZADEH AGHDAM, E., BARZEGARI, A., FARSH DOUSTI HAGH, M., PARVIZI, M., MAHMOUDI AZAR, L., & HEJAZI, M.A.. (2009). CYTOPLASMIC EXPRESSION OF RECOMBINANT INTERLEUKIN-2 AND INTERLEUKIN-4 PROTEINS RESULTS IN HYDROGEN PEROXIDE ACCUMULATION AND REDUCTION IN CATALASE ACTIVITY IN ESCHERICHIA COLI. DARU JOURNAL OF PHARMACEUTICAL SCIENCE, 17(2), 64-71. SID. https://sid.ir/paper/275496/en

Vancouver: Copy

HEJAZI M.S., KARIMI F., MAHDIZADEH AGHDAM E., BARZEGARI A., FARSH DOUSTI HAGH M., PARVIZI M., MAHMOUDI AZAR L., HEJAZI M.A.. CYTOPLASMIC EXPRESSION OF RECOMBINANT INTERLEUKIN-2 AND INTERLEUKIN-4 PROTEINS RESULTS IN HYDROGEN PEROXIDE ACCUMULATION AND REDUCTION IN CATALASE ACTIVITY IN ESCHERICHIA COLI. DARU JOURNAL OF PHARMACEUTICAL SCIENCE[Internet]. 2009;17(2):64-71. Available from: https://sid.ir/paper/275496/en

IEEE: Copy

M.S. HEJAZI, F. KARIMI, E. MAHDIZADEH AGHDAM, A. BARZEGARI, M. FARSH DOUSTI HAGH, M. PARVIZI, L. MAHMOUDI AZAR, and M.A. HEJAZI, “CYTOPLASMIC EXPRESSION OF RECOMBINANT INTERLEUKIN-2 AND INTERLEUKIN-4 PROTEINS RESULTS IN HYDROGEN PEROXIDE ACCUMULATION AND REDUCTION IN CATALASE ACTIVITY IN ESCHERICHIA COLI,” DARU JOURNAL OF PHARMACEUTICAL SCIENCE, vol. 17, no. 2, pp. 64–71, 2009, [Online]. Available: https://sid.ir/paper/275496/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops