EDITORIAL: ARE OTHER FLUORESCENT TAGS USED INSTEAD OF ETHIDIUM BROMIDE SAFER?

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SAEIDNIA SOODABEH; ABDOLLAHI MOHAMMAD

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Journal: DARU Journal of Pharmaceutical Sciences Year:2013 | Volume:21 | Issue:9 Page(s): 1-3

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Information Journal Paper

Title

EDITORIAL: ARE OTHER FLUORESCENT TAGS USED INSTEAD OF ETHIDIUM BROMIDE SAFER?

Author(s)

SAEIDNIA SOODABEH | ABDOLLAHI MOHAMMAD | Issue Writer Certificate

Keywords

ETHIDIUM BROMIDE

CARCINOGENICITY

TRYPANOCIDAL

MUTAGENICITY

MOLECULAR BIOLOGY

Abstract

ETHIDIUM BROMIDE (EtBr) is a well-known fluorescent tag usually applied in molecular biological techniques like agarose gel electrophoresis. The mechanism of action for such compounds is known, in which these compounds are able to bind to the kinetoplastid DNA and to alter their conformation to Z-DNA molecules that stop replication of kinetoplastid DNA leading to Trypanosoma death. Although the usual amounts used in laboratories are considered as below the level required to cause toxicity (LD50 in oral administration in rat is 1.5 g/Kg), the mentioned concentrations are high enough to involve in replication of mitochondrial DNA in some human cell lines. Regarding the points that EtBr is very stable in the environment and if degraded especially by use of bleaches that result in formation of mutagenic compounds, there is a big concern about its use. Although application of EtBr is going to be restricted and replaced with other tags such as SYBRÒ products, the safety of the new substituted compounds are still in doubt and except a few data, there is no essential evidence available to confirm that they are safer than EtBr. Further investigations are recommended to compare their relative biosafety hazards.

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APA: Copy

SAEIDNIA, SOODABEH, & ABDOLLAHI, MOHAMMAD. (2013). EDITORIAL: ARE OTHER FLUORESCENT TAGS USED INSTEAD OF ETHIDIUM BROMIDE SAFER?. DARU JOURNAL OF PHARMACEUTICAL SCIENCE, 21(9), 1-3. SID. https://sid.ir/paper/275751/en

Vancouver: Copy

SAEIDNIA SOODABEH, ABDOLLAHI MOHAMMAD. EDITORIAL: ARE OTHER FLUORESCENT TAGS USED INSTEAD OF ETHIDIUM BROMIDE SAFER?. DARU JOURNAL OF PHARMACEUTICAL SCIENCE[Internet]. 2013;21(9):1-3. Available from: https://sid.ir/paper/275751/en

IEEE: Copy

SOODABEH SAEIDNIA, and MOHAMMAD ABDOLLAHI, “EDITORIAL: ARE OTHER FLUORESCENT TAGS USED INSTEAD OF ETHIDIUM BROMIDE SAFER?,” DARU JOURNAL OF PHARMACEUTICAL SCIENCE, vol. 21, no. 9, pp. 1–3, 2013, [Online]. Available: https://sid.ir/paper/275751/en

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