PRESENCE OF ANTIOXIDANT IN IN VITRO MATURATION MEDIUM AND ITS EFFECTS ON GLUTATHIONE LEVEL, SPINDLE AREA AND RATE OF IN VITRO FERTILIZATION

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MOHAMMADI ROUSHANDEH A.; PASBAKHSH P.; HABIBI ROUDKENAR M.

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Journal: IRANIAN JOURNAL OF MEDICAL SCIENCES (IJMS) Year:2008 | Volume:33 | Issue:1 Page(s): 37-43

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Title

PRESENCE OF ANTIOXIDANT IN IN VITRO MATURATION MEDIUM AND ITS EFFECTS ON GLUTATHIONE LEVEL, SPINDLE AREA AND RATE OF IN VITRO FERTILIZATION

Author(s)

MOHAMMADI ROUSHANDEH A. | PASBAKHSH P. | HABIBI ROUDKENAR M. | Issue Writer Certificate

Keywords

CYSTEAMINE

GSH

MICROTUBULE

MOUS

Abstract

Background: Effect of different doses of CYSTEAMINE on rate of in vitro maturation (IVM), in vitro fertilization (IVF) and glutathione (GSH) level was studied. Metaphase II (MII) spindle area was analyzed for quantification of shape and size of oocytes. Methods: Female mice were primed with 5 IU of pregnant mare's stimulating gonadotrophin. Germinal vesicle (GV) oocytes were retrieved 48 hrs later. IVM medium was supplemented with 0, 50, 100, 200 and 500 mM of CYSTEAMINE. For IVM and IVF assessment in each group, 150 GV oocytes were used. Experiments also included a group of ovulated oocytes (matured in vivo) after priming with pregnant mare's stimulating gonadotrophin and human chorionic gonadotropin. GSH level was measured by 5,5-Dithio-bis (2nitrobenzoic acid) DTNB-GR recycling protocol in GV and MU oocytes. For IVF, MII oocytes were inseminated with mature MOUSe sperm and rate of two-cell embryo was measured. For immunocytochemistry of MICROTUBULE and chromosomes, MII oocytes were fixed by methanol and immunostained with a- and b-microtubule antibody and Hoechst. The spindle area was then analyzed. Results: A dose-dependent improvement was observed in IVM and IVF rate. MU development and two-cell embryo formation were increased significantly in group which received 200 mm CYSTEAMINE compare to the control group.GSH level was increased in presence of CYSTEAMINE in group which received 200 mm CYSTEAMINE. Spindle area was increased in all groups in vitro except for the group which received 500 mm CYSTEAMINE. The difference between spindle area in 200 mm CYSTEAMINE and in vivo group was not significant (P>0.05).Conclusion: Administration of CYSTEAMINE improves IVM and IVF rate in a dose-dependant manner. Also CYSTEAMINE induces glutathione synthesis in MU oocyte and improves MICROTUBULE when administered at a dose of 200 mm. Therefore, addition of CYSTEAMINE as an antioxidant can improve IVM and IVF rate by increasing of oocytes quality.

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APA: Copy

MOHAMMADI ROUSHANDEH, A., PASBAKHSH, P., & HABIBI ROUDKENAR, M.. (2008). PRESENCE OF ANTIOXIDANT IN IN VITRO MATURATION MEDIUM AND ITS EFFECTS ON GLUTATHIONE LEVEL, SPINDLE AREA AND RATE OF IN VITRO FERTILIZATION. IRANIAN JOURNAL OF MEDICAL SCIENCES (IJMS), 33(1), 37-43. SID. https://sid.ir/paper/276164/en

Vancouver: Copy

MOHAMMADI ROUSHANDEH A., PASBAKHSH P., HABIBI ROUDKENAR M.. PRESENCE OF ANTIOXIDANT IN IN VITRO MATURATION MEDIUM AND ITS EFFECTS ON GLUTATHIONE LEVEL, SPINDLE AREA AND RATE OF IN VITRO FERTILIZATION. IRANIAN JOURNAL OF MEDICAL SCIENCES (IJMS)[Internet]. 2008;33(1):37-43. Available from: https://sid.ir/paper/276164/en

IEEE: Copy

A. MOHAMMADI ROUSHANDEH, P. PASBAKHSH, and M. HABIBI ROUDKENAR, “PRESENCE OF ANTIOXIDANT IN IN VITRO MATURATION MEDIUM AND ITS EFFECTS ON GLUTATHIONE LEVEL, SPINDLE AREA AND RATE OF IN VITRO FERTILIZATION,” IRANIAN JOURNAL OF MEDICAL SCIENCES (IJMS), vol. 33, no. 1, pp. 37–43, 2008, [Online]. Available: https://sid.ir/paper/276164/en

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