DETECTION OF 63 KDA PROTEIN IN THE CULTURE SUPERNATANT OF LEISHMANIA TROPICA AND LEISHMANIA MAJOR PROMASTIGOTES

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AMIN LARI M.; ARDEHALI S.; TAHERIAN H.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

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Journal: Iranian Journal of Medical Sciences Year:2001 | Volume:26 | Issue:1-2 Page(s): 74-76

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Title

DETECTION OF 63 KDA PROTEIN IN THE CULTURE SUPERNATANT OF LEISHMANIA TROPICA AND LEISHMANIA MAJOR PROMASTIGOTES

Author(s)

AMIN LARI M. | ARDEHALI S. | TAHERIAN H.

Keywords

LEISHMANIA TROPICA

LEISHMANIAN MAJOR

63 KDA PROTEIN

CULTURE SUPERNATANT

Abstract

Reference strains of LEISHMANIA TROPICA and Leishmania major were recovered from cryopreservation on NNN medium, and adapted to serum-free brain-heart-infusion (BHI) medium. The cultures were centrifuged and supernatants were kept at -200°. The protein concentrations of the CULTURE SUPERNATANTs and extracts were determined and subjected to 20% SDS-PAGE with molecular weight markers. Presence of protein with MW of 63kDa was seen in samples of L. major and L. tropica with no proteins detected in the BHI-only medium. It is not known if the biological functions of the soluble 63 KDA PROTEIN are different from intact GP63 as this molecule has been a candidate for vaccine production.

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APA: Copy

AMIN LARI, M., ARDEHALI, S., & TAHERIAN, H.. (2001). DETECTION OF 63 KDA PROTEIN IN THE CULTURE SUPERNATANT OF LEISHMANIA TROPICA AND LEISHMANIA MAJOR PROMASTIGOTES. IRANIAN JOURNAL OF MEDICAL SCIENCES (IJMS), 26(1-2), 74-76. SID. https://sid.ir/paper/276229/en

Vancouver: Copy

AMIN LARI M., ARDEHALI S., TAHERIAN H.. DETECTION OF 63 KDA PROTEIN IN THE CULTURE SUPERNATANT OF LEISHMANIA TROPICA AND LEISHMANIA MAJOR PROMASTIGOTES. IRANIAN JOURNAL OF MEDICAL SCIENCES (IJMS)[Internet]. 2001;26(1-2):74-76. Available from: https://sid.ir/paper/276229/en

IEEE: Copy

M. AMIN LARI, S. ARDEHALI, and H. TAHERIAN, “DETECTION OF 63 KDA PROTEIN IN THE CULTURE SUPERNATANT OF LEISHMANIA TROPICA AND LEISHMANIA MAJOR PROMASTIGOTES,” IRANIAN JOURNAL OF MEDICAL SCIENCES (IJMS), vol. 26, no. 1-2, pp. 74–76, 2001, [Online]. Available: https://sid.ir/paper/276229/en

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