POLYMERASE CHAIN REACTION, BACTERIOLOGIC DETECTION AND ANTIBIOGRAM OF BACTERIA ISOLATED FROM OTITIS MEDIA WITH EFFUSION IN CHILDREN, SHIRAZ, IRAN

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SHISHEGAR MAHMOOD; FARAMARZI ABOLHASAN; KAZEMI TAYYEBE; BAYAT AKBAR; MOTAMEDIFAR MOHAMMAD

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Journal: IRANIAN JOURNAL OF MEDICAL SCIENCES (IJMS) Year:2011 | Volume:36 | Issue:4 Page(s): 273-280

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Title

POLYMERASE CHAIN REACTION, BACTERIOLOGIC DETECTION AND ANTIBIOGRAM OF BACTERIA ISOLATED FROM OTITIS MEDIA WITH EFFUSION IN CHILDREN, SHIRAZ, IRAN

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Keywords

OTITIS MEDIA WITH EFFUSION

POLYMERASE CHAIN REACTION

ANTIBACTERIAL RESISTANCE

IRAN

ANTIBIOGRAM

Abstract

Background: OTITIS MEDIA WITH EFFUSION is one of the leading causes of hearing loss in children. Effective treatment of effusion in the middle ear requires appropriate empirical treatment and characterization of responsible pathogens. Objective of the present study was to detect pathogens in clinical samples from patients with OTITIS MEDIA WITH EFFUSION in our area and to determine the sensitivity profile of isolated organisms to commonly used antibiotics.Methods: Sixty three samples of middle ear effusion were aseptically obtained from 36 children, who had been treated up to at least two weeks before sampling. They were analyzed using standard bacteriological and multiplex POLYMERASE CHAIN REACTION (PCR) assays. Antibiotic susceptibility tests were also performed.Results: PCR analysis showed that DNA of Streptococcus pneumoniae, Haemophilus influenzae and Moraxella catarrhalis were present in 60 (95.2%) of the samples. The culture-positive effusion for Streptococcus Pneumoniae, Haemophilus Influenzae and Moraxella catarrhaliswas 34.9%.Almost all isolates ofStreptococcus pneumoniae e were sensitive to ciprofloxacin and erythromycin, and none of them was sensitive to co-trimoxazole. None ofH. Influenzae isolates was sensitive to erythromycin, cefixim, co-trimoxazole, ampicillin and amoxicillin. None ofM. Catarrhalis isolates was sensitive to ceftriaxone, co-trimoxazole, ampicillin and amoxicillin.Conclusion: Compared with other studies using PCR method, the number ofH. influenza isolates was in higher in the present study (95.2%). Antibiotic sensitivity profiles of pathogens isolated in this study were different from others. Thus, we can determine empirical antibiotic therapy based on sensitivity profile in our geographic area.

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APA: Copy

SHISHEGAR, MAHMOOD, FARAMARZI, ABOLHASAN, KAZEMI, TAYYEBE, BAYAT, AKBAR, & MOTAMEDIFAR, MOHAMMAD. (2011). POLYMERASE CHAIN REACTION, BACTERIOLOGIC DETECTION AND ANTIBIOGRAM OF BACTERIA ISOLATED FROM OTITIS MEDIA WITH EFFUSION IN CHILDREN, SHIRAZ, IRAN. IRANIAN JOURNAL OF MEDICAL SCIENCES (IJMS), 36(4), 273-280. SID. https://sid.ir/paper/276434/en

Vancouver: Copy

SHISHEGAR MAHMOOD, FARAMARZI ABOLHASAN, KAZEMI TAYYEBE, BAYAT AKBAR, MOTAMEDIFAR MOHAMMAD. POLYMERASE CHAIN REACTION, BACTERIOLOGIC DETECTION AND ANTIBIOGRAM OF BACTERIA ISOLATED FROM OTITIS MEDIA WITH EFFUSION IN CHILDREN, SHIRAZ, IRAN. IRANIAN JOURNAL OF MEDICAL SCIENCES (IJMS)[Internet]. 2011;36(4):273-280. Available from: https://sid.ir/paper/276434/en

IEEE: Copy

MAHMOOD SHISHEGAR, ABOLHASAN FARAMARZI, TAYYEBE KAZEMI, AKBAR BAYAT, and MOHAMMAD MOTAMEDIFAR, “POLYMERASE CHAIN REACTION, BACTERIOLOGIC DETECTION AND ANTIBIOGRAM OF BACTERIA ISOLATED FROM OTITIS MEDIA WITH EFFUSION IN CHILDREN, SHIRAZ, IRAN,” IRANIAN JOURNAL OF MEDICAL SCIENCES (IJMS), vol. 36, no. 4, pp. 273–280, 2011, [Online]. Available: https://sid.ir/paper/276434/en

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